Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 5,897 results for: pcDNA 3.1

Showing: 3181 - 3200 of 5897 results

  5. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

    Enlarge

  6. RF-88-Ate1

    Plasmid
    #79187
    Purpose
    Expression of C-terminally Flag tagged ATE1 in mammalian cells
    Depositor
    Richard Fahlman
    Insert
    ATE1 (ATE1 Human)
    Use
    Tags
    3x Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    79187-plasmid-map-sequence-id-148783

    Enlarge

  7. GLI2 his

    Plasmid
    #87097
    Purpose
    expresses GLI2 in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    GLI2 (GLI2 Human)
    Use
    Tags
    6x His, T7 tag, and Xpress tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    87097-plasmid-map-sequence-id-201477

    Enlarge

  8. RF-227-UB-BMX

    Plasmid
    #79186
    Purpose
    Expression of the C-terminal fragment of BMX that is endogenously generated by caspase cleavage in mammalian cells
    Depositor
    Richard Fahlman
    Insert
    BMX (BMX Human)
    Use
    Tags
    3x Flag and ubiquitin
    Expression
    Mammalian
    Mutation
    delta 1-242
    Promoter
    CMV
    Available since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    79186-plasmid-map-sequence-id-173660

    Enlarge

  14. pORF1-Flag

    Plasmid
    #190565
    Purpose
    Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    ORF1 (from L1.3)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190565-plasmid-map-sequence-id-393221

    Enlarge

  15. SnFR-gamma8

    Plasmid
    #165496
    Purpose
    Green fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.
    Depositor
    Andrew Plested
    Article
    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755
    Insert
    iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    165496-plasmid-map-sequence-id-327724

    Enlarge

  18. pOME0296M

    Plasmid
    #182625
    Purpose
    Reverse sequence of KSHV Kaposincluster-RevCompl-Syn for pcDNA
    Depositor
    Dirk Dittmer
    Insert
    KSHV Kaposincluster_RevCompl_SYN_rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182625-plasmid-map-sequence-id-359444

    Enlarge

  19. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74224-plasmid-map-sequence-id-238509

    Enlarge

  20. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    74223-plasmid-map-sequence-id-334595

    Enlarge

Showing: 3181 - 3200 of 5897 results