162,536 results

1. AICSDP-23:TJP1-mEGFP

   Plasmid

   #87429

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pEJS578_DD-dSpyCas9-mCherry-APEX2

   Plasmid

   #108570

   Purpose

   Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Insert

   dSpyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   APEX2, Destabilization domains, Flag, and mCherry…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF2848

   Plasmid

   #144249

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF21 (TCF21 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-Cdc42-2G

   Plasmid

   #68813

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Lentiviral

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AICSDP-15:ACTB-mEGFP

   Plasmid

   #87425

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   ACTB Homology Arms with mEGFP-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pRSET iGluSnFR3 v857

   Plasmid

   #175186

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v857
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF1034

   Plasmid

   #143745

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   WT1 (WT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EFS-GFP

   Plasmid

   #110834

   Purpose

   positive control for GFP expression

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   elongation factor 1alpha binding sequence (EFS) at GFP promoter region
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-TO-NFIA.1-SOX9

   Plasmid

   #182310

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NFIA-T2A-SOX9 (NFIA, SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-hNurr1-NE

    Plasmid

    #102363

    Purpose

    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

    Depositor

    Shu Leong Ho

    Insert

    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    

    Use

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge