165,075 results

1. 10xHis-3C-BRD4(42-459) BD1-BD2

   Plasmid

   #233886

   Purpose

   pRSF-DUET1 for protein overexpression of human BRD4

   Depositor

   Alessio Ciulli

   Article

   Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   10x his 3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF.111

   Plasmid

   #227187

   Purpose

   pORTMAGE-Ec1 + supplement E. coli SSB

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   CspRecT, mutL_E32K, E. coli SSB
   

   Expression

   Bacterial

   Mutation

   mutL E32K

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDC.109

   Plasmid

   #227189

   Purpose

   T7 IPTG-driven Cas9

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   SpyCas9
   

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRoRL

   Plasmid

   #213132

   Purpose

   Riboptoregulator circuit RoRL for blue-light-activated gene expression

   Depositor

   Andreas Moeglich

   Article

   Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-H2-18 Heavy chain-SpyTag003

   Plasmid

   #216307

   Purpose

   Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP1862 - pAAV-AiE2566m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #214561

   Purpose

   AiE2566m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Bosiljka Tasic, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1 EGFP-Sec61B

   Plasmid

   #207553

   Purpose

   Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-Sec61B
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SSTR4-DuET

   Plasmid

   #213379

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   SSTR4 (SSTR4 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)GαsL-260-NLuc

   Plasmid

   #207158

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(260)N

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB602-TP-mCherry-UnaG

    Plasmid

    #203483

    Purpose

    For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Expression

    Bacterial and Plant

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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