We narrowed to 7,520 results for: Ski;

1. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

   Plasmid

   #172515

   Purpose

   Encodes HvsCas7-11 mammalian expression casette in AAV backbone

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pAAV EFS-HvsCas7-11 for dual vector
   

   Use

   AAV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GAL 103Q+ProGFPp416

   Plasmid

   #15583

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt103Q + Pro (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pMB3-B-TelR

   Plasmid

   #204992

   Purpose

   Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BiP-Myc-KDEL P495L

   Plasmid

   #27166

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Peptide Binding Mutant: P495L

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

   Plasmid

   #101340

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, tat, rev
   

   Use

   Lentiviral

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-c/o NL4-3 Env delta CT

   Plasmid

   #139285

   Purpose

   HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   codon optimized HIV-1 Env delta cytoplasmic tail
   

   Expression

   Mammalian

   Mutation

   deletion of cytoplasmic tail after residue 710

   Promoter

   CMV IE

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-BiP-Myc-KDEL T37G

   Plasmid

   #27165

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   ATP bound form: T37G

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCS2+ Gal4-GBP2-IRES-GBP7-p65

   Plasmid

   #50020

   Purpose

   Multi-purpose expression of a GFP-dependent transcription factor

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
   

   Use

   Multi-purpose expression

   Promoter

   CMV-immediate early promoter and SP6 promoter

   Available Since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MBP-TEVpCS-ADGRB1 CTF-FLAG

   Plasmid

   #217681

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRB1 (ADGRB1 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-926

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX Flag RagC WT

    Plasmid

    #112756

    Purpose

    express Flag-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b_EcSSB_1

    Plasmid

    #78203

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

    Insert

    ssb (G26C)
    

    Expression

    Bacterial

    Mutation

    G26C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe_puro_DEST_Flag_TNIK

    Plasmid

    #45276

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TNIK (TNIK Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_nLightR

    Plasmid

    #217657

    Purpose

    Expresses norepinephrine indicator nLightR under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightR
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT-TO-FH-eGFP

    Plasmid

    #157734

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-eGFP
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-SARS-CoV-2 NSP6

    Plasmid

    #158357

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP6 (ORF1ab )
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pHS1184

    Plasmid

    #205965

    Purpose

    NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH
    

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS NL4-3 envFS eGFP Δrev

    Plasmid

    #101348

    Purpose

    All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta rev

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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