We narrowed to 7,762 results for: loxP

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MTK234_032

Plasmid

#123995

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA1 -hAAVS1

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA3_Cterm-mCherry

Plasmid

#169408

Purpose

Gateway destination vector for cell-free and mamalian expression with C-term mCherry, 8xHis and Myc tag

Depositor

Kirill Alexandrov

Type

Empty backbone

Use

Gateway Cloning

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Publication

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Expression

Insect

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GGE368

Plasmid

#120737

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Antibiotic resistance marker for selection after transformation in Y. lipolytica (Marker_Nat)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

Marker_Nat

Use

Cloning/assembly plasmid

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEx_Hsp70::EGFPpA-ttBtagRFPt

Plasmid

#48877

Purpose

PhiC31 exchange vector with the Hsp70::EGFPpA insert. Selection via EGFP to tagRFPt-color switch in the heart

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al Development. 2013 Sep 18.

Insert

HSP70::EGFPpA

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

Plasmid

#205283

Purpose

GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)

Depositor

Matthias Prigge

Insert

GCaMP8f

Use

AAV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYL322d1/Vn-C

Plasmid

#183154

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

Depositor

Yao-Guang Liu, Qinlong Zhu

Publication

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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52 pLIB_3C_C_STREP

Plasmid

#160300

Purpose

C-term 3C cleavable 2xStrepII tag for insect cell expression

Depositor

John Weir

Publication

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII

Expression

Insect

Promoter

polyhydrin

Available since

Nov. 15, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_014

Plasmid

#123929

Purpose

Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBIattB destination vector

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPI464

Plasmid

#113216

Purpose

generic knockin vector for C-terminal tagging with mScarlet

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mScarlet

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Publication

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Expression

Insect

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pKM377

Plasmid

#134210

Purpose

Baculovirus expression of CENP-HKM and His-CENP-I

Depositor

Iain Cheeseman

Publication

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-H/K/M untagged + his-I

Expression

Insect

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Publication

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Expression

Insect

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Publication

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINEneo

Plasmid

#53536

Purpose

pTriex-based expression vector which adds a C-his8 tag to the inserted gene

Depositor

Ray Owens

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pKM499

Plasmid

#134216

Purpose

Baculovirus expression of CENP-U CENP-Q CENP-OP

Depositor

Iain Cheeseman

Publication

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPU + CENPQ+ CENPOP

Expression

Insect

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBS5

Plasmid

#83799

Purpose

Useful for tagging yeast proteins with N-terminal CFP tag; G418 selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Expression

Yeast

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pTW073

Plasmid

#64754

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a V5 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Publication

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Publication

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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