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pFB2

Plasmid

#195282

Purpose

Bicistronic DTR and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Publication

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR 2A Puro (HBEGF Synthetic, Human)

Expression

Mammalian

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR1-tdTomato

Plasmid

#124235

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR1-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-LARP7

Plasmid

#113545

Purpose

Produces an N-terminal GST-tagged full length LARP7 protein.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

LARP7 (LARP7 Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Publication

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Promoter

8xGliBS-delta-crystallin minimal promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-CX4.3-7aa-mClover3

Plasmid

#74260

Purpose

Mammalian expression of C-terminal fusion of mClover3 to CX43

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Connexin 43

Tags

mClover3

Expression

Mammalian

Promoter

CAG and CMV

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAR2A

Plasmid

#23789

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR2A (PRKAR2A Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pUBQ:Citrine-TurboID-3xMyc

Plasmid

#199244

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Publication

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID-3xMyc

Tags

TurboID-3xMyc

Expression

Plant

Promoter

Arabidopsis UBQ10 promoter with intron

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3 2 kb prom. and exon 1 fragment CD274

Plasmid

#107007

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Publication

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV gfaABC1D NES-jRCaMP1a

Plasmid

#171120

Purpose

Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

Depositor

Thomas Oertner

Publication

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jRCaMP1a

Use

AAV

Tags

His Tag, T7 Tag, XpressTag

Expression

Mammalian

Promoter

gfaABC1D

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-LaminB1-10

Plasmid

#58020

Purpose

Localization: Nuclear Envelope, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mTagBFP2-Endosomes-14

Plasmid

#55293

Purpose

Localization: Endosomes, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Endosomes (RHOB Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits
DUSP6

Plasmid

#40772

Depositor

Dustin Maly

Publication

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

DUSP6 (DUSP6 Human)

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSfi-HspLacZ

Plasmid

#33351

Depositor

David Kingsley, Doug Mortlock

Publication

Mortlock et al Genome Res. 2003 Sep;13(9):2069-81. Epub

Insert

Hsp68 promoter (Hspa1a Mouse)

Use

Mouse Targeting

Tags

LacZ

Expression

Mammalian

Promoter

Hsp68 (Hspa1a)

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pDONR223-PIP5K2A

Plasmid

#23744

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K2A (PIP4K2A Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-MAP3K11

Plasmid

#23473

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K11 (MAP3K11 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-LOC91807

Plasmid

#23546

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC91807 (MYLK3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP2K2

Plasmid

#23555

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K2 (MAP2K2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pDONR223-PRPS1

Plasmid

#23588

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRPS1 (PRPS1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA h-MLSN

Plasmid

#21083

Depositor

Craig Montell

Publication

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TrpM1 (TRPM1 Human)

Tags

Myc

Expression

Mammalian

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pINDUCER22-MKK6E-Flag

Plasmid

#99259

Purpose

Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

Depositor

Alejandro Adam, Peter Vincent

Publication

Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

Insert

MKK6E (MAP2K6 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S207E T211E

Promoter

TRE2

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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