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pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Tags

His tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

Plasmid

#140003

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pB-CuO-ZNF729-HA-CymR-Puro

Plasmid

#248164

Purpose

Expresses ZNF729 upon cumate induction

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

ZNF729 (ZNF729 Human)
HA-CymR-T2A-puro

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-MP2

Plasmid

#36097

Purpose

3rd generation lentiviral plasmid

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 puro

Plasmid

#98290

Purpose

Variant of lentiCRISPRv2 that confers puromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Article

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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spNW30

Plasmid

#173485

Purpose

Expresses spNW30 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW30

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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