We narrowed to 13,238 results for: BASE

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pU6-sgGAL4-4

Plasmid

#46916

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-4
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-FLuc

Plasmid

#170575

Purpose

Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

Luc

Use

Luciferase and Retroviral

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBOBI-FLuc

Plasmid

#170674

Purpose

Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

luc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV-F

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ-CBE-Cas9n-Act3.0

Plasmid

#178955

Purpose

It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

Plasmid

#100831

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-2dV-Camui (T305D/T306D)

Plasmid

#220368

Purpose

T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Synthetic, Rat)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Mutation

changed both Threonine 305 and 306 to aspartic ac…

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pUAP1

Plasmid

#63674

Purpose

Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

Depositor

Nicola Patron

Article

Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

Insert

RFP cloning selection cassette

Use

Synthetic Biology

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Article

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Expression

Bacterial

Promoter

sppA

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-TRPM7

Plasmid

#23476

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRPM7 (TRPM7 Human)

Use

Gateway Cloning

Mutation

Insert encodes NCBI: NR_149154.2 bases 265 to 146…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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