Skip to main content
Addgene

We narrowed to 5,415 results for: PEP;

Showing: 3221 - 3240 of 5415 results
  1. pMSCVpuro-Mito-DsRed1

    Plasmid
    #87379
    Purpose
    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
    Depositor
    Insert
    TXN2 mitochondrial transit peptide (TXN2 Human)
    Use
    Retroviral
    Tags
    DsRed1
    Expression
    Mammalian
    Mutation
    First 60 aa only
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3-sACE2v2.4(732)-8h

    Plasmid
    #154103
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)
    Depositor
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-G…
    Promoter
    CMV
    Available since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3-sACE2v2(732)-8h

    Plasmid
    #154102
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)
    Depositor
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3-sACE2v6-Fc(IgA1)

    Plasmid
    #145161
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6
    Depositor
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgA1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pMWCAVI_H6BAP-OROV_N

    Plasmid
    #240162
    Purpose
    Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli
    Depositor
    Insert
    Nucleoprotein
    Use
    Tags
    6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 3221 - 3240 of 5415 results