We narrowed to 12,322 results for: BASE
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Plasmid#176679PurposeExpression of Drosophila codon optimized spCas9 under Drosophila Act5C promoterDepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprTagsExpressionInsectMutationDrosophila codon optimizedPromoterD. melanogaster Act5CAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
1477 pcDNA3 flag HA Akt1
Plasmid#9021DepositorInsertAkt1 (AKT1 Human)
UseTagsHA and flagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-VIM
Plasmid#227301PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.DepositorInsertsgRNA Targeting C-terminus of VIM (VIM Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorInsertGαq-LgB97 (GNAQ Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQ79
Plasmid#175064Purposeexpresses mutant of the huntingtin exon1 in mammalian cellsDepositorInsertpolyQ79 (HTT Human)
UseTagsExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-RacFRET-U6ac-ctrl guides
Plasmid#168247Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertRacFRET
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI #2
Plasmid#139666PurposeEndogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1011-luc2
Plasmid#227106PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1011DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1014-luc2
Plasmid#227108PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1014DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GalT-mCherry
Plasmid#214271PurposeMammalian expression of GalT-mCherry (Golgi marker)DepositorInsertGalT(1-82)-mCherry (B4GALT1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_18xTEAD_luc2
Plasmid#173003Purposefirefly luciferase reporter vector with 18x clustered TEAD binding sitesDepositorInsert18xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro
Plasmid#209646PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-Q2L7-T2176C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro
Plasmid#209642PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro
Plasmid#209641PurposeTo install m.G3635 mutation on mtDNADepositorInsertL17-FZY2-S100C
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Dlg4-HaloTag KI
Plasmid#139656PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorInsertgRNA and HaloTag donor
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160-754*
Plasmid#123274PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncationDepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1063-luc2
Plasmid#227109PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1063DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176N-UGI-Puro
Plasmid#209649PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176N
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM162
Plasmid#173221PurposeExpresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1DepositorInsertsScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
MCP-mCherry-GFP11-GB1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)
Plasmid#105200PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(HC)-P2A-antiPD1(LC)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-CBh-eGFP
Plasmid#206885Purposecloning backbone for sgRNA using OptScf2DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Bsn-GFP KI
Plasmid#139665PurposeEndogenous tagging of Bassoon: C-terminal (amino acid position: F3938)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-GFP
Plasmid#58245PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFPDepositorInsertsattR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP
UseLentiviral and RNAi; Gateway destionation vectorTagsGFP, NLS, T2A, and TetRExpressionMammalianMutationPromoterSFFV and noAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-L1T3D
Plasmid#33276DepositorUseTagsExpressionMammalianMutationPromoterT7Available sinceJan. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt-3HA
Plasmid#39284DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3HA
Plasmid#39285DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-TM
Plasmid#111844PurposeMammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tetherDepositorInsertHIV-1 Env (BG505 SOSIP.664)
UseTagsCD5 leader sequence and TM helix from MHC class IExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1065-luc2
Plasmid#227111PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1065DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1094-luc2
Plasmid#227114PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-MYO1C
Plasmid#227306PurposeDonor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)DepositorInsertMYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1397 DddAtox C
Plasmid#158097Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1397 DddAtox N
Plasmid#158094Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-STK4
Plasmid#172989Purposeconstitutive expression of FLAG-tagged STK4DepositorInsert3xFLAG-STK4 (STK4 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC3
Plasmid#172996Purposeconstitutive expression of FLAG-tagged WWC3DepositorInsert3xFLAG-WWC3 (WWC3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
SnapTag-Binder
Plasmid#179142PurposeBinder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).DepositorInsertSspB
UseTagsSNAP-tagExpressionMammalianMutationY11K/A15E, residue numbering from pdb: 1ou9PromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mTurquoise2
Plasmid#111254PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasmDepositorInsertTorA(sp)-mTurquoise2
UseTagsExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Bsn KI
Plasmid#139664PurposeEndogenous tagging of Bassoon: N-terminal (amino acid position: L7)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only