We narrowed to 5,365 results for: puc

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pTFE3-donor

Plasmid

#112377

Purpose

CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

Depositor

Kevin White

Insert

TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)

Use

CRISPR

Mutation

hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7xKS-sfGFP

Plasmid

#117394

Purpose

Tn7 tagging vector pTn7xKS with sfGFP expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pARNTL-donor

Plasmid

#112384

Purpose

CRISPR donor plasmid to tag human transcription factor ARNTL with GFP

Depositor

Kevin White

Insert

ARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)

Use

CRISPR

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCap-SG5-Apr

Plasmid

#227579

Purpose

Capture plasmid with apramycin resistance and pSG5 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCap-101-Apr

Plasmid

#227507

Purpose

Capture plasmid with apramycin resistance and pIJ101 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Puro

Plasmid

#207726

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi-BN

Plasmid

#60518

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

Plasmid

#194728

Purpose

AAVS1-tdTomato targeting vector, compartible with Cpf1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

tdTomato

Use

CRISPR; Knockin targeting plasmid

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pT2ST

Plasmid

#59384

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-dfrA

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Blast

Plasmid

#207709

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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MAPT_Halo_C_allele

Plasmid

#178143

Purpose

Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (MAPT Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pC0042-PguCas13b crRNA backbone

Plasmid

#103853

Purpose

For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-PguCas13b DR-BbsI-BbsI-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-3xHA

Plasmid

#207696

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCE1

Plasmid

#174434

Purpose

Candida optimized monomeric eGFP with RIPLING linker sequence.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

Candida optimized monomeric GFP

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShHELIX-KanR (CJT70)

Plasmid

#181793

Purpose

pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Puro

Plasmid

#207729

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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