164,853 results

1. SETD2(SET)-Cas9

   Plasmid

   #186700

   Purpose

   Plasmid encoding SETD2-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   SETD2(SET)-Cas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBL101

   Plasmid

   #199722

   Purpose

   A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_Ef1a_DIO_hTyrHA_minBack

   Plasmid

   #205286

   Purpose

   Induces NM-like pigment accumulation in catecholaminergic neurons

   Depositor

   Matthias Prigge

   Insert

   hTyrosinase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pODN-mNG-ANLN

   Plasmid

   #183834

   Purpose

   Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN homology arms with mNeonGreen-linker (ANLN Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3124

   Plasmid

   #144600

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGT-B1

   Plasmid

   #122579

   Purpose

   MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZac2.1 gfaABC1D-Glt1-eGFP

   Plasmid

   #176862

   Purpose

   Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Glt1-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGT-Ah9

   Plasmid

   #122576

   Purpose

   MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   tetQ/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. USP15

   Plasmid

   #28282

   Depositor

   Cheryl Arrowsmith

   Insert

   USP15 (USP15 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   Contains dusp and Ubl domains (residues 6-223).

   Promoter

   Available Since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

10. MSCB-hDot1Lmut

    Plasmid

    #74174

    Purpose

    For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    hDot1L 163 GSG to RCR mutation*

    Promoter

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only