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We narrowed to 9,474 results for: BLI;

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Showing: 6481 - 6520 of 9474 results

  1. pFRT-TODestRFP_HuD

    Plasmid
    #65766
    Purpose
    expresses RFP-tagged HuD in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL4 (ELAVL4 Human)
    Expression
    Mammalian
    Mutation
    V23M mutation in RFP
    Promoter
    CMV, 2x Tet
    Available Since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. RHPMP133 (mOrange2-beta Actin)

    Plasmid
    #88837
    Purpose
    Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    beta-Actin (ACTB Human)
    Tags
    mOrange2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88837-plasmid-map-sequence-id-177529

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  3. pFRT-DestFLAGHA_HuB

    Plasmid
    #65758
    Purpose
    constitutively expresses FLAG-HA-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Tags
    FLAG HA
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available Since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

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  5. pFRT-TODestGFP_HuB

    Plasmid
    #65761
    Purpose
    expresses GFP-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available Since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65761-plasmid-map-sequence-id-115012

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  6. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid
    #41878
    Depositor
    Anna Huttenlocher
    Insert
    photoactivatable Rac1 (RAC1 Human)
    Use
    Zebrafish expression
    Tags
    6x His and mCherry
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Available Since
    Oct. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    41878-plasmid-map-sequence-id-56671

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  7. pNIC28-hKdm2b-CxxC-PHD

    Plasmid
    #49216
    Purpose
    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.
    Depositor
    Rob Klose
    Insert
    Kdm2b (KDM2B Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    49216-plasmid-map-sequence-id-72771

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  8. GPX3

    Plasmid
    #38899
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX3 (GPX3 Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. COVID-SARS2 NSP16/10

    Plasmid
    #159615
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10
    Depositor
    Opher Gileadi
    Inserts
    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    Tags
    His6-Zb-TEV and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159615-plasmid-map-sequence-id-311066

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  10. pDest eGFP CUGBP1

    Plasmid
    #61276
    Purpose
    Mammalian expression of EGFP-tagged human CUGBP1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    CUG-BP (CELF1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61276-plasmid-map-sequence-id-99587

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  13. Hs.NRAS Q61L

    Plasmid
    #83178
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61L
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83178-plasmid-map-sequence-id-158141

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  15. pMT-HA-STX1

    Plasmid
    #89310
    Purpose
    Expresses HA tagged syntaxin 1 in Drosophila cells
    Depositor
    Andrew Peden
    Insert
    STX1 (Syx1A Fly)
    Tags
    HA tag
    Expression
    Insect
    Promoter
    P-MT (Metallothionein)
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89310-plasmid-map-sequence-id-190170

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  16. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid
    #78336
    Purpose
    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX3 (PAX3 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Promoter
    TRE2
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pTXB1-CII

    Plasmid
    #51566
    Purpose
    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cII
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51566-plasmid-map-sequence-id-78825

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  18. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61K
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83180-plasmid-map-sequence-id-158143

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  19. Hs.HRAS G12D

    Plasmid
    #83183
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12D
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83183-plasmid-map-sequence-id-158146

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  20. pUG-amdSYM-mak10TR

    Plasmid
    #75433
    Purpose
    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae
    Depositor
    John McCusker
    Inserts
    MAK10TR
    ApR
    amdSYM
    Expression
    Yeast
    Mutation
    N-terminal 740 bp of MAK10 ORF
    Promoter
    Native promoter and PTEF
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75433-plasmid-map-sequence-id-142819

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  21. Hs.HRAS Q61R

    Plasmid
    #83188
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61R
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83188-plasmid-map-sequence-id-158151

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  22. pLenti-cytoMetGFP

    Plasmid
    #37561
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    only contains cytoplasmic domain amino acids K956…
    Promoter
    CMV
    Available Since
    Aug. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    37561-plasmid-map-sequence-id-47323

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  23. NEK2

    Plasmid
    #39163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NEK2 (NEK2 Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    T175A inactivating mutant
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pDONR221-CTSB

    Plasmid
    #158443
    Purpose
    Gateway Cloning compatible entry vector for the human CTSB gene.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    CTSB (CTSB Human)
    Use
    Gateway entry vector
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158443-plasmid-map-sequence-id-308885

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  25. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    SRF (SRF Human)
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. pAAV-DIO-EF1a-TVA-P2A-EYFP

    Plasmid
    #177017
    Purpose
    For expression of the avian TVA receptor in a cre-dependent manner
    Depositor
    Peter Jonas
    Insert
    TVA-P2A-EYFP (CD320 Coturnix japonica)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177017-plasmid-map-sequence-id-353740

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  27. 4H4H

    Plasmid
    #51314
    Depositor
    Cheryl Arrowsmith
    Insert
    4H4H (EHMT1 4H4H, Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pMT-TAK1-V5-His

    Plasmid
    #63753
    Purpose
    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TGF-β activated kinase 1 (Tak1 Fly)
    Tags
    V5-His
    Expression
    Insect
    Promoter
    pMT
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63753-plasmid-map-sequence-id-106438

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  29. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  30. pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)

    Plasmid
    #219654
    Purpose
    Neuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.
    Depositor
    Thomas Oertner
    Insert
    2xLyn-ERex-mKate2-bPAC(F198Y)
    Use
    AAV
    Tags
    ER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)
    Expression
    Mammalian
    Promoter
    Syn
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    219654-plasmid-map-sequence-id-434848

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  31. MARS

    Plasmid
    #205232
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205232-plasmid-map-sequence-id-404748

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  32. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  33. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Eleanor Chen
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83966-plasmid-map-sequence-id-160487

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  34. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  35. HPGD

    Plasmid
    #39154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HPGD (HPGD Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. tet-pLKO.puro_shGAC

    Plasmid
    #110423
    Purpose
    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110423-plasmid-map-sequence-id-213314

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  37. pAG416-GAL-hnRNPA1

    Plasmid
    #181710
    Purpose
    Galactose inducible expression of hnRNPA1
    Depositor
    Alejandro Chavez
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Expression
    Yeast
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181710-plasmid-map-sequence-id-430953

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  38. isoform PKC Iota (JCE597)

    Plasmid
    #182030
    Purpose
    yeast expression of mouse PKC Iota with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Iota (Prkci Mouse)
    Expression
    Mammalian
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182030-plasmid-map-sequence-id-361960

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  39. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  40. GOT1

    Plasmid
    #39022
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GOT1 (GOT1 Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 6481 - 6520 of 9474 results