Skip to main content
Addgene
Showing: 6481 - 6520 of 8371 results

  2. EPB41

    Plasmid
    #32040
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPB41 (EPB41 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    FERM domain only (amino acids 1-279)
    Promoter
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. HSD17B8

    Plasmid
    #38853
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B8 (Hsd17b8 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

    Enlarge

  5. GHRHR

    Plasmid
    #39173
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GHRHR (GHRHR Human)
    Use
    Baculovirus expression
    Tags
    Signal-His6-TEV
    Expression
    Mutation
    N20->Q mutation of putative glycosylsation sit…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Hs.KRAS4b Q61K

    Plasmid
    #83164
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61K
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83164-plasmid-map-sequence-id-158127

    Enlarge

  8. pAdTrack-mLIP

    Plasmid
    #49197
    Purpose
    adenoviral shuttle vector with mouse C/EBPbeta LIP fragment
    Depositor
    Jed Friedman
    Insert
    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    only the LIP fragment
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. Myc-gamma2s-EGFP

    Plasmid
    #118040
    Purpose
    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG2 (Gabrg2 Rat)
    Use
    Tags
    EGFP and cMyc epitope EQKLISEEDL inserted between…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118040-plasmid-map-sequence-id-228639

    Enlarge

  11. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid
    #110378
    Purpose
    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    crystallin zeta (CRYZ Human)
    Use
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110378-plasmid-map-sequence-id-213403

    Enlarge

  12. pDNR-hSox2

    Plasmid
    #49389
    Purpose
    donor vector for cloning human Sox2 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Expression
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    none
    Available since
    Nov. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. Mouse Alpha2 delta-2 mutMIDAS HA pMT2

    Plasmid
    #58733
    Purpose
    expression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cells
    Depositor
    Annette Dolphin
    Insert
    alpha2 delta-2 (Cacna2d2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    MIDAS site mutations (D300VSGS to AVAGA)
    Promoter
    SV40
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58733-plasmid-map-sequence-id-90818

    Enlarge

  14. pJK571

    Plasmid
    #75379
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glycine-209 to aspartate and synthetic ge…
    Promoter
    n/a
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    75379-plasmid-map-sequence-id-142756

    Enlarge

  16. Hs.KRAS4b K104A

    Plasmid
    #83152
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K104A mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    K104A
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83152-plasmid-map-sequence-id-158115

    Enlarge

  17. Hs.KRAS4b K104Q

    Plasmid
    #83153
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K104Q mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    K104Q
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83153-plasmid-map-sequence-id-158116

    Enlarge

  18. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid
    #50888
    Purpose
    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50888-plasmid-map-sequence-id-76361

    Enlarge

  19. GRM3 (3SM9)

    Plasmid
    #32870
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    His tag with TEV cleavage and Honeybee melittin S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. ABL2

    Plasmid
    #39178
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABL2 (ABL2 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Truncated (see comments)
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. CUL5

    Plasmid
    #38975
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CUL5 (CUL5 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Mouse Cul5 (GI:239051067) with two point mutation…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid
    #82528
    Purpose
    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1-84 with mutation of RKR70-72, K75, …
    Promoter
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82528-plasmid-map-sequence-id-156824

    Enlarge

  23. Hs.KRAS4b G12F

    Plasmid
    #83149
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12F mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12F
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83149-plasmid-map-sequence-id-158112

    Enlarge

  24. Hs.KRAS4b S181E

    Plasmid
    #83138
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S181E mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S181E
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83138-plasmid-map-sequence-id-158101

    Enlarge

  25. Hs.KRAS4b S181D

    Plasmid
    #83137
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S181D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S181D
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83137-plasmid-map-sequence-id-158100

    Enlarge

  26. Hs.KRAS4b S181A

    Plasmid
    #83136
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S181A mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    S181A
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83136-plasmid-map-sequence-id-158099

    Enlarge

  27. pDNR-hAkt1

    Plasmid
    #49387
    Purpose
    donor vector for cloning human Akt1 with 6xHN tag
    Depositor
    Randall McKinnon
    Insert
    Akt1 (AKT1 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN (6 repeating His-Asn subunits)
    Expression
    Mutation
    tga to cca at nt 1725 with respect to GenBank ID …
    Promoter
    none
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. GUCY1A3

    Plasmid
    #39120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GUCY1A3 (GUCY1A1 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. GUCY1B3

    Plasmid
    #39035
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GUCY1B3 (GUCY1B1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. HSD17B14

    Plasmid
    #39160
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B14 (HSD17B14 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. BBOX1

    Plasmid
    #39176
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BBOX1 (BBOX1 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. pTRE2_CMV_Mex3a_t2a_mCherry

    Plasmid
    #223244
    Purpose
    tetO responsive expression of Mex3a in mammalian cells with t2a cleaved mCherry
    Depositor
    Stavros Lomvardas
    Insert
    Mex3a (Mex3a Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    amino acids conserved, underlying DNA sequence re…
    Promoter
    CMV
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    223244-plasmid-map-sequence-id-441777

    Enlarge

  37. pLEX_307-SARS-CoV-2-NSP3-V5

    Plasmid
    #214429
    Purpose
    Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in mammalian cells
    Depositor
    Mohan Babu
    Insert
    SARS-CoV-2 NSP3 (part of SARS-CoV-2 ORF1ab) (ORF1ab )
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    214429-plasmid-map-sequence-id-437257

    Enlarge

  38. pLD-puro-Cc-IFIT5_WT-VA

    Plasmid
    #191221
    Purpose
    Lentiviral expression of human IFIT5_WT in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191221-plasmid-map-sequence-id-433869

    Enlarge

  39. pLD-puro-Cc-SARS-CoV-2_NSP3-VA

    Plasmid
    #191214
    Purpose
    Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells
    Depositor
    Mohan Babu
    Insert
    Non structural protein 3 (ORF1ab SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191214-plasmid-map-sequence-id-431857

    Enlarge

  40. pLD-puro-Cc-SARS-CoV-2_NSP16-VA

    Plasmid
    #191215
    Purpose
    Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells
    Depositor
    Mohan Babu
    Insert
    Non structural protein 16 (ORF1ab SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191215-plasmid-map-sequence-id-422110

    Enlarge

Showing: 6481 - 6520 of 8371 results