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LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C3006

Plasmid

#91096

Purpose

Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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HB501: pMVP (L1-L4) RIP

Plasmid

#121688

Purpose

pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Rat Ins1 promoter (RIP; β-cell specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IF101: pMVP (L1-L4) EF1a promoter

Plasmid

#121687

Purpose

pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 WT

Plasmid

#123083

Purpose

Gateway entry clone encoding human GABARAPL1 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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HB401: pMVP (L1-L4) CMV promoter

Plasmid

#121686

Purpose

pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_B2615

Plasmid

#91078

Purpose

Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BsaI ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

AtU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 WT

Plasmid

#123086

Purpose

Gateway entry clone encoding human GABARAPL2 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2813

Plasmid

#91092

Purpose

Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A G120A

Plasmid

#123070

Purpose

Gateway entry clone encoding human MAP1LC3A G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_B2517

Plasmid

#91074

Purpose

Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

TaU3

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120A

Plasmid

#123073

Purpose

Gateway entry clone encoding human MAP1LC3B G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 G116A

Plasmid

#123087

Purpose

Gateway entry clone encoding human GABARAPL2 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 116 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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