162,586 results

1. pHEN2-SG4

   Plasmid

   #196071

   Purpose

   Express wild type SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4
   

   Use

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAKTaq

   Plasmid

   #25712

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   David Engelke

   Article

   Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

   Insert

   Taq polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. circRNA-synIRES-R25-mNeonGreen

   Plasmid

   #188115

   Purpose

   Template plasmid to transcribe circular RNA encoding mNeonGreen

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3b-8XGliBS:EGFP

   Plasmid

   #84602

   Purpose

   Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

   Depositor

   James Chen

   Article

   Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   8X Gli-binding site

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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