162,325 results

1. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. loxP-G418-LoxP-TurboID-Rab11 HR

   Plasmid

   #230026

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTEF2

   Plasmid

   #43971

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF

   Available since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pBAD.Sigma32.I54N

   Plasmid

   #59982

   Purpose

   Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

   Depositor

   Jeffery Kelly

   Article

   Zhang et al ACS Chem Biol. 2014 Jul 22.

   Insert

   Sigma32 I54N
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I54N

   Promoter

   Available since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV sMTase (eGFP)

   Plasmid

   #89930

   Purpose

   Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC-IRES-MN
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   deactivated Cas9

   Promoter

   CMV

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psPAX2-D64V-NC-PP7

   Plasmid

   #122945

   Purpose

   PP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particles

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   NC-PCP (PP7 coat protein) fusion protein in Gag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D64V

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe EGFR(L858R/T790M)

   Plasmid

   #32073

   Depositor

   Matthew Meyerson

   Article

   Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L858R/T790M

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJMP2846

   Plasmid

   #160676

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-Nsp12

    Plasmid

    #165117

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp12 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge