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pDIV151

Plasmid

#226180

Purpose

Expresses Cas9 in a backbone compatible with K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

Cas9

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Mutation

Promoter

GAP1

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-HOXB5-DD-hygro

Plasmid

#222582

Purpose

PiggyBac transposon plasmid for Shield1-inducible expression of BCL2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-HOXB5

Plasmid

#222584

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXB5, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-UBB

Plasmid

#222569

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of UBB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

UBB (UBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC6

Plasmid

#222566

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC6

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC6 (HDAC6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TET1

Plasmid

#222572

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of TET1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

TET1 (TET1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RB1

Plasmid

#222550

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RB1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RB1 (RB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOXA10

Plasmid

#222539

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXA10

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXA10 (HOXA10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB3

Plasmid

#222540

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB3 (HMGB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-BCL2

Plasmid

#222583

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BCL2, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BCL2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ESX1

Plasmid

#222563

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ESX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ESX1 (ESX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ121-mTCABE-T

Plasmid

#218173

Purpose

Express plastid monomeric TALE-linked CABE-T, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a TadA-derived deaminase, and UGI

Depositor

Yiping Qi

Article

Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

Insert

TALE-Intron-TadAC-DddA full (E1347A)-UGI

Use

Plastid base editing

Tags

Expression

Plant

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN

Plasmid

#208866

Purpose

Stably express GFP-tagged OPTN for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT56

Plasmid

#218727

Purpose

Expresses N-terminally EGFP-tagged IFT56 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nozaki et al J Cell Sci. 2016 Dec 7. pii: jcs.197004.

Insert

IFT56 (TTC26 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 2

Plasmid

#207608

Purpose

sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

acccccaaacctgactgact

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Duet_OPTN-thrombin-GST

Plasmid

#216843

Purpose

For expression in E. coli of OPTN (Internal ref. SMC1789)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

Optineurin (OPTN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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