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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Mito-PyronicSF/pCMV-myc-mito

Plasmid

#124813

Purpose

Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

Depositor

Luis Felipe Barros

Publication

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PyronicSF

Tags

Mitochondrial Sequence Signal

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Publication

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MaLionR

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Publication

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Expression

Bacterial

Promoter

sppA

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-FNLS-P2A-Puro

Plasmid

#110841

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pHR-FUSN-mCh-Cry2olig

Plasmid

#101224

Purpose

FUS(1-214) fused to mCh-Cry2olig

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2olig

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTP943

Plasmid

#104157

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fab specific nanobody TP886

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Publication

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTetON-EGFP

Plasmid

#89453

Purpose

Tet/Dox-inducible expression of EGFP in mammalian cells (control)

Depositor

Brad Zuchero

Publication

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Green cGull

Plasmid

#86867

Purpose

Green fluorescent probe for cGMP

Depositor

Tetsuya Kitaguchi

Publication

Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Green cGull

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Publication

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Expression

Bacterial

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.cTNT.iCre

Plasmid

#69916

Purpose

Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

Depositor

William Pu

Publication

Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

Has service

AAV9

Insert

improved cre (iCre) recombinase and tdTomato

Use

AAV

Tags

The iCre coding frame and tdTomato coding frame i…

Promoter

Chicken cardiac troponin T

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GAL4UAS-Luciferase reporter

Plasmid

#64125

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAL4UAS

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GAL4UAS

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCAG_smFP FLAG

Plasmid

#59756

Purpose

encodes multiple copies of an epitope tag FLAG

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

smFP _FLAG

Expression

Mammalian

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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