We narrowed to 8,877 results for: BLI;

1. kiCAP-AAV-MyoAAV1C

   Plasmid

   #224438

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDLSTP insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-SspB-HaloTag-ITSN1-DHPH

   Plasmid

   #176128

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. kiCAP-AAV-MyoAAV2E

   Plasmid

   #224441

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDQGYQ insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. kiCAP-AAV-MyoAAV3D

   Plasmid

   #224445

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYREL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. kiCAP-AAV-MyoAAV3E

   Plasmid

   #224446

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDHGVL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. kiCAP-AAV-MyoAAV4B

   Plasmid

   #224449

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYTSV insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. kiCAP-AAV-MyoAAV4D

   Plasmid

   #224451

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDHGVL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. kiCAP-AAV-MyoAAV4C

   Plasmid

   #224450

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYTSM insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. kiCAP-AAV-MyoAAV3C

   Plasmid

   #224444

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDYSSV insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. kiCAP-AAV-MyoAAV3B

    Plasmid

    #224443

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDYSGL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid

    #225947

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S3E S17E S19E

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid

    #225948

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S3A S17A S19A

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AID-C12*-BE4max

    Plasmid

    #216729

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225703

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225697

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plentiCRISPRv2-PURO-LYSET-gRNA1

    Plasmid

    #210489

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA1 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plentiCRISPRv2-PURO-LYSET-gRNA2

    Plasmid

    #210490

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA2 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SspB-HaloTag-DHPH-ITSN1

    Plasmid

    #176113

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223-H-RAS V12

    Plasmid

    #31201

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    H-Ras (HRAS Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    G12V constitutively active

    Promoter

    Available since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225698

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K27M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K27M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SspB-iRFP-DHPH-ITSN1

    Plasmid

    #176117

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid

    #194190

    Purpose

    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    5 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid

    #194193

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 899)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTO_Snf7_LAP

    Plasmid

    #101851

    Purpose

    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    SNF7 (SNF7 Budding Yeast)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Bidirectional Vector

    Plasmid

    #237067

    Purpose

    Express LgBiT-KRAS(G12V)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    LgBiT and SmBiT

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Bidirectional Vector

    Plasmid

    #237066

    Purpose

    Express LgBiT-KRAS (WT)_SmBiT-CRAF-RBD Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    LgBiT and SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. cRAF CRD-RBD: KRAS G12C BiBRET Vector

    Plasmid

    #236872

    Purpose

    Express cRAF CRD-RBD: KRAS G12C in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD)-CRD (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. Nluc-KRAS(G12D)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid

    #236867

    Purpose

    Express NanoLuc(R)-KRAS(G12D)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. Nluc-KRAS(G12C)-cRAF(FL)-HaloTag BiBRET Vector

    Plasmid

    #236868

    Purpose

    Express NanoLuc(R)-KRAS(G12C)-cRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. Nluc-KRAS(G12C)-cRAF(RBD)-HaloTag BiBRET Vector

    Plasmid

    #236869

    Purpose

    Express NanoLuc(R)-KRAS(G12C)-cRAF(RBD)-HaloTag(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    cRAF(RBD) (RAF1 Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    G12C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. NanoBRET BiBRET Vector HaloTag-KRAS 2B WT-BRAF RBD-NanoLuc

    Plasmid

    #236853

    Purpose

    Express HaloTag(R)-KRAS 2B WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    KRAS (KRAS Human)
    BRAF (RBD) (BRAF Human)
    

    Use

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

    Plasmid

    #225956

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Full-length CKAP4 with additional luminal domain …

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid

    #225939

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    STIM1 (STIM1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid

    #225944

    Purpose

    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    ATL1 (ATL1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AID-C12*-n'Cas9-BE4max

    Plasmid

    #216730

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-n'Cas9-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSCBE3-NG-HF1

    Plasmid

    #218160

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSCBE3-NG-Hypa

    Plasmid

    #218161

    Purpose

    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AID-C12*-dCas9-BE4max

    Plasmid

    #216731

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-dCas9-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. An R57A, R59A CAAX pMT2

    Plasmid

    #206119

    Purpose

    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. An R57A, R59A HA CAAX pMT2

    Plasmid

    #206126

    Purpose

    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid

    #194195

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 732)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid

    #194196

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 884)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid

    #194197

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 449)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid

    #194191

    Purpose

    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    4 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid

    #194192

    Purpose

    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    3 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGL3-pSMANTIS_1kb_KLF2mut 548

    Plasmid

    #194194

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 548)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. SspB-mCherry-DHPH-ITSN1

    Plasmid

    #176103

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. P521_VSX2_p2A-h2b_mRuby3

    Plasmid

    #239104

    Purpose

    Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

    Depositor

    Karl Wahlin

    Article

    Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

    Insert

    VSX2 (VSX2 Human)
    

    Use

    CRISPR

    Tags

    -p2A-h2b-mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous VSX2 promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLenti-CMV_ACE2-Flag_Hygro

    Plasmid

    #191998

    Purpose

    Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. CLYBL-TO-hNIL-BSD-mApple

    Plasmid

    #124230

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Insert

    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge