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pCAG-N-CretrcintG

Plasmid

#69572

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - N-CretrcintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic active lipin 1

Plasmid

#32007

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

Promoter

CMV, SP6

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pBS mNFATc3-EE

Plasmid

#17868

Depositor

Jerry Crabtree

Article

Graef et al Cell. 2001 Jun 29. 105(7):863-75.

Insert

NFATc3 (Nfatc3 Mouse)

Tags

EE

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#191797

Purpose

vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized dCas13d

Use

CRISPR

Expression

Mammalian

Mutation

R239A, H244A, R858A, H863A

Promoter

CBh, SV40, hU6

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PERK 7: PERK.K618A.9E10.pBABE

Plasmid

#21820

Depositor

David Ron

Insert

PERK K618A (Eif2ak3 Mouse)

Use

Retroviral

Tags

Myc (9E10)

Expression

Mammalian

Mutation

kinase dead K618A mutant

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV

Plasmid

#45062

Purpose

Expression of CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV (Camk4 Mouse)

Expression

Mammalian

Mutation

*

Promoter

RSV

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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SID4x-dCas9-KRAB

Plasmid

#106399

Purpose

Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Inserts

SID4x (MXD1 Human)
KRAB (ZNF10 Human)

Tags

FLAG, HA, and NLS

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1 (AAVA)

Plasmid

#14957

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Three Serine to Alanine mutations in the C-termin…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pMVA2RBS035

Plasmid

#121051

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway Cloning

Mutation

Insert encodes truncated protein relative to NP_0…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Flag Snail 2SA

Plasmid

#16219

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

2SA: S97A, S101A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 EGFP-Sumo2

Plasmid

#13385

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 (SUMO2 Human)

Tags

EGFP and GST

Expression

Bacterial

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-SENP6m

Plasmid

#18716

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

FLAG-SENP6m (SENP6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C1030A

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pGL3-mFgf21pro1-luc

Plasmid

#101797

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Nix

Plasmid

#100795

Purpose

Mammalian expression of Nix (Bnip3L)

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST27-alpha-catenin

Plasmid

#20139

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pDEST27-alpha catenin

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only