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PEP107E

Plasmid

#97393

Purpose

binary construct of Golgi-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Tags

ER target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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CeNL-H2B_pcDNA3

Plasmid

#89511

Purpose

Cyan color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDRFLIP37

Plasmid

#65515

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-Cer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDRFLIP36

Plasmid

#65514

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-Cer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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PEP103E

Plasmid

#97389

Purpose

binary construct of plastid-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plastid target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PEP105E

Plasmid

#97391

Purpose

binary construct of peroxisome-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Peroxisome target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCyclinB1(L45A,R42A)-mCherry (1963)

Plasmid

#39852

Depositor

Jonathon Pines

Article

Gavet et al J Cell Biol. 2010 Apr 19;189(2):247-59.

Insert

CyclinB1 (CCNB1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

L45A and R42A

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pMC-1-PvUbi2-6

Plasmid

#197706

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi2 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAG25-L-IFP-F[1] (clon 3)

Plasmid

#52899

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Vector for homologous recombination

Promoter

TEF promoter

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
pAG32-L-IFP-F[2] (clon 3)

Plasmid

#52900

Purpose

Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Vector for homologous recombination

Promoter

TEF promoter

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pDRFLIP43

Plasmid

#65519

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edAFPt9-attR1 and attR2-edCer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAIO-LbCpf1

Plasmid

#137847

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDRFLIP39

Plasmid

#65517

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-15

Plasmid

#119994

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-22

Plasmid

#120001

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-11

Plasmid

#119990

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D.R

Plasmid

#51510

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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