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We narrowed to 13,014 results for: cdna

Showing: 6561 - 6600 of 13014 results

  4. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  5. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid
    #126444
    Purpose
    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated
    Depositor
    Jeremy Wilusz
    Article
    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:
    Insert
    Fndc3b (Fndc3b Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    circFndc3b exons are separated by a miniature int…
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126444-plasmid-map-sequence-id-245088

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  7. pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)

    Plasmid
    #49063
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49063-plasmid-map-sequence-id-72185

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  12. YFP(159-238)-gamma-7 in pcDNAI/Amp

    Plasmid
    #54473
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(159-238)-gamma-7 (GNG7 Aequorea victoria, Human)
    Use
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-7 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54473-plasmid-map-sequence-id-115045

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  21. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (159-238)-Beta 1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55592-plasmid-map-sequence-id-114863

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  22. pcDNA 3.1(-) rat C/EBP beta (LIP)

    Plasmid
    #12562
    Depositor
    Peter Johnson
    Insert
    CCAAT/Enhancer-binding Protein beta LIP (Cebpb Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Daniel Durocher
    Article
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Insert
    RNF168 (RNF168 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133983-plasmid-map-sequence-id-261913

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  26. YFP(159-238)Stop in pcDNA1/amp

    Plasmid
    #60891
    Purpose
    This vector attaches YFP(159-238) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    YFP(159-238)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    60891-plasmid-map-sequence-id-112575

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Showing: 6561 - 6600 of 13014 results