Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,133 results for: gnas

Clear keywords
Showing: 8201 - 8250 of 10133 results

  2. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid
    #140451
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140451-plasmid-map-sequence-id-291874

    Enlarge

  3. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

    Enlarge

  14. pOCC92

    Plasmid
    #118910
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available Since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    118910-plasmid-map-sequence-id-234552

    Enlarge

  15. CD4_LD 0_CS G_tTA-BFP

    Plasmid
    #58869
    Purpose
    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58869-plasmid-map-sequence-id-94230

    Enlarge

  23. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

    Enlarge

  25. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Available Since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. mCherry_LD 0_CS M_tTA-BFP

    Plasmid
    #58874
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    58874-plasmid-map-sequence-id-94235

    Enlarge

  28. mCherry_LD 0_CS A_tTA-BFP

    Plasmid
    #58873
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58873-plasmid-map-sequence-id-94234

    Enlarge

  33. pVCS7

    Plasmid
    #231621
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231621-plasmid-map-sequence-id-491218

    Enlarge

  34. pTARGEX-APO

    Plasmid
    #242446
    Purpose
    Targets high levels of recombinant protein to apoplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    N-terminal fusion with PR1b signal peptide
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242446-plasmid-map-sequence-id-490468

    Enlarge

  36. pTARGEX-ER

    Plasmid
    #242449
    Purpose
    Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    ER-retrieval motif (KDEL) and Signal peptide deri…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242449-plasmid-map-sequence-id-489545

    Enlarge

  38. SP_C3

    Plasmid
    #228159
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast (strain ATCC 204508 / S288c) PIR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q03178_PIR1_YEAST:0-18
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228159-plasmid-map-sequence-id-451652

    Enlarge

  39. pVCS1

    Plasmid
    #231615
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231615-plasmid-map-sequence-id-464094

    Enlarge

  40. pVCS9

    Plasmid
    #231623
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231623-plasmid-map-sequence-id-464087

    Enlarge

  41. pVCS8

    Plasmid
    #231622
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231622-plasmid-map-sequence-id-464071

    Enlarge

  42. pVCS6

    Plasmid
    #231620
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231620-plasmid-map-sequence-id-464093

    Enlarge

  43. pVCS5

    Plasmid
    #231619
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231619-plasmid-map-sequence-id-464091

    Enlarge

  44. pVCS4

    Plasmid
    #231618
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231618-plasmid-map-sequence-id-464053

    Enlarge

  45. pVCS3

    Plasmid
    #231617
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231617-plasmid-map-sequence-id-464096

    Enlarge

  46. pVCS2

    Plasmid
    #231616
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al ACS Synth Biol. 2025 Jan 17;14(1):193-20
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pvan/Pcym
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231616-plasmid-map-sequence-id-464095

    Enlarge

  47. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210734
    Purpose
    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Frodo sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    210734-plasmid-map-sequence-id-464595

    Enlarge

  50. pET22b Ag43 160N 6H mCherry-R5 AmpR

    Plasmid
    #225141
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 160N, mCherry
    Tags
    Ag43 protein fused to silaffin-R5 and mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225141-plasmid-map-sequence-id-460500

    Enlarge

Showing: 8201 - 8250 of 10133 results