We narrowed to 4,788 results for: AAT

1. pTorPE-R-GECO1

   Plasmid

   #32465

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Use

   Lab constructed

   Tags

   6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

   Expression

   Bacterial

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBO.ACT006

   Plasmid

   #182716

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   GFP in cloning site of crRNA for easier screening…

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. tet_pLKO.1_puro_shLuc

   Plasmid

   #136587

   Purpose

   Expresses an inducible short hairpin targeting firefly luciferase sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-RacFRET-U6ac-ctrl guides

   Plasmid

   #168247

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMP-EZH2_3

   Plasmid

   #36389

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   EZH2 (EZH2 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

6. ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173202

   Purpose

   Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKO.1 puro p53 shRNA 2

   Plasmid

   #10672

   Depositor

   William Hahn

   Article

   Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

   Insert

   p53 shRNA (TP53 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFD5-U6-3-t-attP40

   Plasmid

   #133561

   Purpose

   gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   attP40 region guide RNAs
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MASTL B4.4 gRNA

   Plasmid

   #90755

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MASTL

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   MASTL (Guide Designation B4.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330.sgNf1.4

    Plasmid

    #92028

    Purpose

    pX330 sgNf1.4 for Nf1 deletion

    Depositor

    Wen Xue

    Article

    Song et al Gastroenterology. 2017 Apr;152(5):1161-1

    Insert

    sgNf1.4
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pXPR_BRD003 DLAT-2

    Plasmid

    #184485

    Purpose

    Lentivirus gRNA targeting human DLAT gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    DLAT-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVUTHshGATA1-tTR-KRAB

    Plasmid

    #11650

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. DKC1 F1.3 gRNA

    Plasmid

    #90652

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DKC1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DKC1 (Guide Designation F1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330.Pten.b

    Plasmid

    #66588

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. PX458_AHR_1

    Plasmid

    #101076

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelper-T7IS1

    Plasmid

    #140631

    Purpose

    Expresses ShCAST under the T7 promoter and an empty sgRNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSaPIPLC His

    Plasmid

    #173810

    Purpose

    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Mary F Roberts, Anne Gershenson

    Article

    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

    Insert

    Staphylococcus aureus plc
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-C1-FAK-HA-V744G

    Plasmid

    #35040

    Depositor

    Anna Huttenlocher

    Article

    Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

    Insert

    focal adhesion kinase (Ptk2 Mouse)
    

    Use

    Tags

    2x HA and mCherry

    Expression

    Mammalian

    Mutation

    GFP-FAK-V744G was generated from GFP-FAK using th…

    Promoter

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_AHR_2

    Plasmid

    #101077

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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