We narrowed to 6,930 results for: CAD
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Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
UseTags3xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags1xHA tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCherryGFP-Inframe-noFSE
Plasmid#177619PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).DepositorInsertNone
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-WT
Plasmid#177614PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshiftingDepositorInsertSARS-CoV-2 FSE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-TET-GFP-FF3
Plasmid#11662Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 MCV LTco S220A
Plasmid#112193PurposeExpression of MCPyV LT mutant (Ser 220 to Ala)DepositorInsertMCPyV LT codon optimized
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDicer
Plasmid#89144PurposeExpression of hDicer in Sf9 CellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt1
Plasmid#49186PurposecDNA of Akt1 with myristyolation signal in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11
Plasmid#172068PurposeProtein trap plasmid for inserting GFP11 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
UseTagsN-myristoylationExpressionInsectMutationPromoterGAL4-responsive UAS hsp70Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-E2419K
Plasmid#19994DepositorInsertmammalian target of rapamycin-E2419K (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Glutamine 2419 to LysinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG103QDProGFPp303
Plasmid#15587DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsFLAG and GFPExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52546PurposeTargeting vector for homologous recombination in mouse ES cells; Rosa26 locusDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-FF3
Plasmid#11663Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1-Orai3
Plasmid#73563PurposeGreen fluorescent reporter of Orai3-associated calcium influxDepositorInsertG-GECO1-Orai3 (ORAI3 Human, Synthetic)
UseTagsG-GECO1ExpressionMammalianMutationPromoterCMV Immdeiate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
UseTagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastMutationPromoternoAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5E
Plasmid#171948PurposeExpresses INPP5E in mammalian cellsDepositorInsertINPP5E (INPP5E Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only