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pSKAP_AI

Plasmid

#122711

Purpose

pSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AI

Expression

Bacterial

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AH

Plasmid

#122710

Purpose

pSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AH

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AG

Plasmid

#122709

Purpose

pSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AG

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSKAP_AE

Plasmid

#122707

Purpose

pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AE

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAW188

Plasmid

#113178

Purpose

E. coli expression vector for untagged E. coli Cas1 R131A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R131A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHES944

Plasmid

#112642

Purpose

express SST2 from the GAL1 promoter

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

SST2

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAW167

Plasmid

#113187

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 R40E

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R40E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW156

Plasmid

#113185

Purpose

E. coli expression vector for untagged E. coli Cas1 K259E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K259E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW186

Plasmid

#113184

Purpose

E. coli expression vector for untagged E. coli Cas1 R163A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R163A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW151

Plasmid

#113183

Purpose

E. coli expression vector for untagged E. coli Cas1 R146A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R146A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW150

Plasmid

#113182

Purpose

E. coli expression vector for untagged E. coli Cas1 S143A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

S143A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW174

Plasmid

#113181

Purpose

E. coli expression vector for untagged E. coli Cas1 R138A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW189

Plasmid

#113180

Purpose

E. coli expression vector for untagged E. coli Cas1 Q136A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

Q136A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW185

Plasmid

#113179

Purpose

E. coli expression vector for untagged E. coli Cas1 R132A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R132A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW184

Plasmid

#113176

Purpose

E. coli expression vector for untagged E. coli Cas1 R112A + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R112A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW152

Plasmid

#113175

Purpose

E. coli expression vector for untagged E. coli Cas1 K12E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

K12E

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW158

Plasmid

#113177

Purpose

E. coli expression vector for untagged E. coli Cas1 R112E + Cas2

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2

Expression

Bacterial

Mutation

R112E

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaGSPdR

Plasmid

#108205

Purpose

PdR

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin reductase

Expression

Bacterial

Promoter

lac promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHPro54G

Plasmid

#108204

Purpose

4th Pro5

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Pro-Pro-Pro-Pro-Pro

Expression

Bacterial

Promoter

lac promoter

Available Since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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