We narrowed to 10,838 results for: ENA

1. pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

   Plasmid

   #125225

   Purpose

   Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSilencer2.1-U6-NL1F50

   Plasmid

   #190831

   Purpose

   For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   NL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. J23103_GB

   Plasmid

   #65991

   Purpose

   MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Constitutive promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. J23107_GB

   Plasmid

   #65999

   Purpose

   MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Constitutive promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. J23116_GB

   Plasmid

   #66003

   Purpose

   MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Constitutive promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

   Plasmid

   #119074

   Purpose

   Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:MCP-p65-HSF1-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

   Plasmid

   #119075

   Purpose

   Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   ubb:Com-KRAB-2a-Cerulean
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish ubiquitin promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVC-Ds-DrU6a:sgRNA-com-Ds

   Plasmid

   #119077

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xCom-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVC-Ds-DrU6a:sgRNA-MS2-Ds

   Plasmid

   #119076

   Purpose

   To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   U6a:2xMS2-sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish U6a promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQCas12j2

    Plasmid

    #189780

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Insert

    Cas12j2
    

    Use

    CRISPR; Gateway compatible cas12j2 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

    Plasmid

    #83484

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1048A, H1053A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R, R883K, R887A

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. G1397 DddAtox-C–dSpCas9

    Plasmid

    #157836

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TO-mCherry-N

    Plasmid

    #210271

    Purpose

    N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDA_791

    Plasmid

    #216079

    Purpose

    Minimal plasmid; contains only ori and ampR, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Destination

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA295

    Plasmid

    #215986

    Purpose

    Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; SV40NLS_v1.19
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AA014

    Plasmid

    #216057

    Purpose

    Fragmid fragment: (2A Selection) Puromycin resistance

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    P2A_v1.1; PuroR_v1.1; Stop
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA163

    Plasmid

    #216061

    Purpose

    Fragmid fragment: (2A Selection) Stop only

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Stop
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA065

    Plasmid

    #215965

    Purpose

    Fragmid fragment: (Pol 2 promoter) human EF1a promoter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    EF1a_v1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEAST-pBen-sfGFP

    Plasmid

    #114598

    Purpose

    "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    PBen

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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