We narrowed to 7,329 results for: GAN

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SOX2 KO (T2A-H2B-EGFP)

Plasmid

#167969

Purpose

SOX2 CDS replacement with T2A-H2B-EGFP

Depositor

Emma Rawlins

Publication

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 2 (SOX2 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

Plasmid

#127365

Purpose

Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)

Plasmid

#29438

Depositor

Joan Conaway, Ronald Conaway

Publication

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.) E653Q

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-fCX3CL1

Plasmid

#164421

Purpose

AAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptors

Depositor

Connie Cepko

Publication

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

Cx3cl1 (Cx3cl1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Publication

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del

Plasmid

#30528

Depositor

Gerhart Ryffel

Publication

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

HNF1B (HNF1B Human)

Use

Cre/Lox; Flp/frt

Tags

Myc

Expression

Mammalian

Mutation

P328L329delCCTCT

Available since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

Plasmid

#29439

Depositor

Joan Conaway, Ronald Conaway

Publication

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) with Q1116K mutation

Available since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

Plasmid

#29437

Depositor

Joan Conaway, Ronald Conaway

Publication

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) E653Q

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA5FRT/Flag-hINO80 HSA (212-526)

Plasmid

#29443

Depositor

Joan Conaway, Ronald Conaway

Publication

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 212-526 (a.a.)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A

Plasmid

#127790

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TAC-EB-C5

Plasmid

#80485

Purpose

piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Publication

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Publication

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Mutation

G12V

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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5mCUC1 pGreenII0129

Plasmid

#12067

Depositor

David Bartel

Publication

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pCS2 Notch1 LNG (CC→SS)-6MT

Plasmid

#41739

Depositor

Raphael Kopan

Publication

Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

Insert

Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 leader peptide (aa 1-23) …

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pBpuro - HA KLF4 FL delta K5S

Plasmid

#34592

Depositor

Michael Ruppert

Publication

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

LTR

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pCM3.12

Plasmid

#19606

Depositor

Geraldine Seydoux

Insert

spe-11 ORF+3' in pDONR201 (spe-11 Nematode)

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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F40F8.11 RNAi

Plasmid

#186731

Purpose

Knockdown of F40F8.11 gene

Depositor

Seung-Jae Lee

Insert

F40F8.11 targeting RNA

Use

RNAi

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCM3.07

Plasmid

#19603

Depositor

Geraldine Seydoux

Insert

pal-1 ORF+3' in pDONR201 (pal-1 Nematode)

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCM3.10

Plasmid

#19604

Depositor

Geraldine Seydoux

Insert

pgl-3 ORF+3' in pDONR201 (pgl-3 Nematode)

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pJW4.04

Plasmid

#171209

Purpose

bacterial expression of MBP-tagged PGL-3

Depositor

Geraldine Seydoux

Publication

Schmidt et al bioRxiv 2020.10.15.340570

Insert

pgl-3 (pgl-3 Nematode)

Tags

MBP

Expression

Bacterial

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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