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Flag-FOXF1

Plasmid

#153122

Purpose

Expression of Flag tagged human FOXF1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXF1 (FOXF1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEY17

Plasmid

#191023

Purpose

flp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18 CELE_Y48D7A.2 (flp-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pvimentin-emiRFP670

Plasmid

#136572

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-mCherry2-G4

Plasmid

#164167

Purpose

Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDA025

Plasmid

#131384

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A En

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBHM2404

Plasmid

#173441

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-NAT1

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGF-mSox2-ONL

Plasmid

#65719

Purpose

mSox2 reporter plasmid (expresses ONL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Sox2 promoter and ONL

Use

Lentiviral and Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDA034

Plasmid

#131386

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

D702Y RT

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-CyclinF-ΔF

Plasmid

#32973

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

FLAG

Expression

Mammalian

Mutation

F-box mutations: L35A and P36A

Promoter

CMV

Available Since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only
pyc126m

Plasmid

#158777

Purpose

ribosome-tagged GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-FOXI1

Plasmid

#153126

Purpose

Expression of Flag tagged human FOXI1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXI1 (FOXI1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

Plasmid

#128607

Purpose

Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

Plasmid

#128608

Purpose

Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pTfR-PATagRFP

Plasmid

#31946

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

TfR

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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gh123

Plasmid

#106790

Purpose

expression of gRNA targeting ST8SIA3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA3 (ST8SIA3 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pyc111s

Plasmid

#158781

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6s-RPL10a

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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