We narrowed to 5,897 results for: dna plasmids

1. pRDA_889

   Plasmid

   #216080

   Purpose

   ITRs from AAV2, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   ITRs from AAV2
   

   Use

   AAV; Destination

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-FHC-POLQ-DM

   Plasmid

   #64880

   Purpose

   lentiviral expression of human POLQ double mutant (in POL and HDL domains)

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   K121M and D2330A,Y2331A

   Promoter

   EF1alpha

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD068

   Plasmid

   #136471

   Purpose

   SSB-GFP IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-GFP (ssb E. coli)
   

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SECURE miniABEmax-V82G (pJUL1828)

   Plasmid

   #131313

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SuExp His-Myc-mPLD4

   Plasmid

   #201185

   Purpose

   Protein expression plasmid for recombinant His-Myc tag mouse PLD4

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD4 (Pld4, Pld4, Pld4 Mouse)
   

   Tags

   His-Myc, Factor Xa cleavable

   Expression

   Mammalian

   Mutation

   cytosolic and transmembrane domain truncated; add…

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD072

   Plasmid

   #136472

   Purpose

   SSB-C-term-GFP fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-C-term-GFP (ssb E. coli)
   

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP attached to C-terminus at Phe 178

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV.U6.gDNMT1.Cas9-T2A-GFP

   Plasmid

   #170362

   Purpose

   Plasmid used for Crispr/cas9 based disruption of human DNMT1.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Available Since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.175

   Plasmid

   #184996

   Purpose

   Express -Eco1 HEK3 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK3 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCL.177

   Plasmid

   #184998

   Purpose

   Express -Eco1 EMX1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   EMX1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. QSOX1-luciferase

    Plasmid

    #113352

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP9A-luciferase

    Plasmid

    #113354

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ATP9A-enhancer (ATP9A Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.180

    Plasmid

    #185001

    Purpose

    Express -Eco1 AAVS1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    AAVS1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCHECK2-miR-34 MT

    Plasmid

    #78259

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated miR-34 target site
    

    Use

    Luciferase; Microrna activity

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CCGT to GG…

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTE4254

    Plasmid

    #89050

    Purpose

    Expresses St chimeric gRNA and human codon-optimized StCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    St chimeric gRNA
    hStCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1-FHC-POLQ-S1977P

    Plasmid

    #64876

    Purpose

    lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    S1977P, mimicking the chaos1 mutation

    Promoter

    EF1alpha

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. SunTagCACTA1g2-22aa-TET1cd

    Plasmid

    #106437

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFETCh_KMT2B

    Plasmid

    #101070

    Purpose

    Donor vector for 3' FLAG tag of human KMT2B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KMT2B homology arms (KMT2B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Promoter

    EF1a and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZJQIBEBT003

    Plasmid

    #140053

    Purpose

    The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

    Depositor

    Jia Zhang

    Article

    Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

    Insert

    eMutS-L157C-G233C-CBM-EGFP
    

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    L157C, G233C

    Promoter

    T7

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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