We narrowed to 10,818 results for: HA

1. FLAG-MARK3-GK-CR

   Plasmid

   #229424

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK3-GK-CR (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gatekeeper mutation M132G; silent mutation CRISPR…

   Promoter

   EFS

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-MARK2-GK-CR

   Plasmid

   #229423

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   MARK2-GK-CR (MARK2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gatekeeper mutation M129G; silent mutation CRISPR…

   Promoter

   EFS

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgTAZ-R-2

   Plasmid

   #229449

   Purpose

   knockout of WWTR1 (TAZ)

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgTAZ-R-2 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgTAZ-R-1

   Plasmid

   #229448

   Purpose

   knockout of WWTR1 (TAZ)

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgTAZ-R-1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgCDK1-ROSA

   Plasmid

   #229443

   Purpose

   knockout of CDK1

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgCDK1-ROSA (CDK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgMARK2-3-3

   Plasmid

   #229442

   Purpose

   knockout of MARK2 and MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-3-3 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgMARK2-3-2

   Plasmid

   #229441

   Purpose

   knockout of MARK2 and MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-3-2 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgMARK2-3-1

   Plasmid

   #229440

   Purpose

   knockout of MARK2 and MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK2-3-1 (MARK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgMARK3-R-3

   Plasmid

   #229439

   Purpose

   knockout of MARK3

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   sgMARK3-R-3 (MARK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6;bU6;EFS

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sgMARK3-R-1

    Plasmid

    #229437

    Purpose

    knockout of MARK3

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK3-R-1 (MARK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sgMARK2-R-3

    Plasmid

    #229436

    Purpose

    knockout of MARK2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK2-R-3 (MARK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgMARK2-R-2

    Plasmid

    #229435

    Purpose

    knockout of MARK2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK2-R-2 (MARK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgMARK2-R-1

    Plasmid

    #229434

    Purpose

    knockout of MARK2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgMARK2-R-1 (MARK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid

    #226989

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 1a to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid

    #226991

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2a to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

    Plasmid

    #226987

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A6 to create OPA1-R194G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

    Plasmid

    #226986

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A7 to create OPA1-R194G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid

    #226992

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2b to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid

    #226990

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 1b to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

    Plasmid

    #226988

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A9 to create OPA1-R194G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. METTL3

    Plasmid

    #155725

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    METTL3 (METTL3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    H330Q compared to BC052244, no mutations compared…

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EDC3

    Plasmid

    #155561

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    EDC3 (EDC3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pIG-724pUC19-tNGFR-P2A-EVER2

    Plasmid

    #186105

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    EVER2 (TMC8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIG-734pUC19-tNGFR-P2A-PIK3R1

    Plasmid

    #186107

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIG-721pUC19-tNGFR-P2A-RhoH

    Plasmid

    #186116

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    RHOH (RHOH Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIG-720pUC19-tNGFR-P2A-ZAP70

    Plasmid

    #186120

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pIG-735pUC19-tNGFR-P2A-PIK3CD

    Plasmid

    #186087

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIG-723pUC19-tNGFR-P2A-EVER1

    Plasmid

    #186088

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    EVER1 (TMC6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIG-727pUC19-tNGFR-P2A-WASP

    Plasmid

    #186089

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    WASP (WAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIG-714pUC19-tNGFR-P2A-SAP

    Plasmid

    #186091

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    SAP (SH2D1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIG-728pUC19-tNGFR-P2A-WIP

    Plasmid

    #186094

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    WIP (WIPF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIG-729pUC19-tNGFR-P2A-Moesin

    Plasmid

    #186095

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    MOESIN (MSN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIG-742pUC19-tNGFR-P2A-Caspase8

    Plasmid

    #186099

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CASPASE8
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIG-705pUC19-tNGFR-P2A-STAT5b

    Plasmid

    #186103

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT5B (STAT5B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTR-460 tNGFR-P2A-STAT4

    Plasmid

    #186075

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT4 (STAT4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTR-458 tNGFR-P2A-STAT2

    Plasmid

    #186076

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT2 (STAT2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTR-455 tNGFR-P2A-WAS

    Plasmid

    #186082

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    WAS (WAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTR-459 tNGFR-P2A-STAT6

    Plasmid

    #186070

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT6 (STAT6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT22UbhaEng2aE4PCh(#271)

    Plasmid

    #184085

    Purpose

    cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2a-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pT22UbhaEng2bE4PCh(#272)

    Plasmid

    #184086

    Purpose

    cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    3HA-ZfEng2b-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge