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pMC8b-X2up

Plasmid

#176162

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site X-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-X2dw

Plasmid

#176163

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site X-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-BASU-EGFP

Plasmid

#163845

Purpose

In vitro transcription of BASU-EGFP. Parton lab clone JRR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

In vitro transcription of rna

Tags

EGFP

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4A

Plasmid

#145366

Purpose

Cell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4A

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS4B

Plasmid

#145367

Purpose

Cell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS2A

Plasmid

#145363

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

His and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLMB708

Plasmid

#83782

Purpose

Tartrate biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL0996 containing all of RL0997

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB717

Plasmid

#83788

Purpose

Erythritol biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL90085 containing all of pRL90087

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS25Att

Plasmid

#98526

Purpose

BB1 with insert: RPS25Att

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS25Att

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCPA2-C-18

Plasmid

#54175

Purpose

Localization: Actin Capping Protein , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

MCPA2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
CtkA_D155Q_pET21a

Plasmid

#27430

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
CtkA_D179Q_pET21a

Plasmid

#27431

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
DH-BB[5'SIN;181/25ORF]

Plasmid

#242479

Purpose

Helper plasmid to produce CHIKV-pseudotyped propogation incompetent Sindbis virus

Depositor

Justus Kebschull

Article

Kim et al bioRxiv 2025.06.25.661405; doi: https://

Insert

Chikungunya virus 181/25 strain structural proteins

Use

Sindbis virus helper construct

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_sfGFP-TurboID

Plasmid

#240226

Purpose

Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

sfGFP-TurboID

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-PDGFRStalk-TM

Plasmid

#240243

Purpose

Lentiviral transfer plasmid encoding IL-13 with PDGFR stalk and TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV27

Plasmid

#237947

Purpose

Encodes TRBV27 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV27-TRAC (TRBV27 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV2

Plasmid

#237903

Purpose

Encodes TRBV2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV2-TRAC (TRBV2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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ExRai-PKNAR(T/A)

Plasmid

#226632

Purpose

Negative-control mutant for ExRai-PKNAR biosensor

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

ExRai-PKNAR (T/A)

Expression

Mammalian

Mutation

Phosphorylatable Thr mutated to Ala

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AG

Plasmid

#182155

Purpose

Expression of chimeric AG (N-mApple-intron-C-eGFP)

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AG

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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