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We narrowed to 4,138 results for: LYC

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Showing: 3301 - 3320 of 4138 results

  2. PF3D7_1136200-bio

    Plasmid
    #47730
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PF3D7_1136200
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin replaced…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47730-plasmid-map-sequence-id-68870

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  3. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid
    #156419
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156419-plasmid-map-sequence-id-308855

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  9. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  10. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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  12. EBL1-bio

    Plasmid
    #47792
    Purpose
    Expresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised EBL1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47792-plasmid-map-sequence-id-68762

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  13. pLenti PGK Puro GFP-GABARAPL1 G116A

    Plasmid
    #123245
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 116 to Alanine
    Promoter
    PGK
    Available Since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    123245-plasmid-map-sequence-id-245084

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  20. pDEST-CMV 3xFLAG-LC3B-G120A-GFP

    Plasmid
    #123108
    Purpose
    Expresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123108-plasmid-map-sequence-id-244364

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Showing: 3301 - 3320 of 4138 results