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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,590 results for: sequence

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Showing: 3301 - 3320 of 13590 results

  1. pAc5.1B-lambdaN-HA-DmDIS3_AA

    Plasmid
    #148209
    Purpose
    Insect Expression of DmDIS3
    Depositor
    Elisa Izaurralde
    Insert
    DmDIS3 (Dis3 Fly)
    Expression
    Insect
    Mutation
    7 silent mutations compared to the sequence given…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148209-plasmid-map-sequence-id-290657

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  2. pAc5.1B-lambdaN-HA-DmMUB-E_AA

    Plasmid
    #148216
    Purpose
    Insect Expression of DmMUB-E
    Depositor
    Elisa Izaurralde
    Insert
    DmMUB-E
    Expression
    Insect
    Mutation
    two silent mtations and an insertion of 4 AA (91W…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148216-plasmid-map-sequence-id-290664

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  3. pT7-V5-SBP-C1-HsXRN1_1-1173_V

    Plasmid
    #147754
    Purpose
    Mammalian Expression of HsXRN1_1-1173
    Depositor
    Elisa Izaurralde
    Insert
    HsXRN1_1-1173 (XRN1 Human)
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147754-plasmid-map-sequence-id-290201

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  4. pnEA-NpM-HsTOB1_Q

    Plasmid
    #147315
    Purpose
    Bacterial Expression of HsTOB1
    Depositor
    Elisa Izaurralde
    Insert
    HsTOB1 (TOB1 Human)
    Expression
    Bacterial
    Mutation
    one non silent mutation F309L compared to the seq…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147315-plasmid-map-sequence-id-289762

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  5. pnEA-NpM-HsTOB1_1-138_Q

    Plasmid
    #147316
    Purpose
    Bacterial Expression of HsTOB1_1-138
    Depositor
    Elisa Izaurralde
    Insert
    HsTOB1_1-138 (TOB1 Human)
    Expression
    Bacterial
    Mutation
    one silent mutation compared to teh sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147316-plasmid-map-sequence-id-289763

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  6. pLNHA-C1-HsTOB1_Q

    Plasmid
    #147286
    Purpose
    Mammalian Expression of HsTOB1
    Depositor
    Elisa Izaurralde
    Insert
    HsTOB1 (TOB1 Human)
    Expression
    Mammalian
    Mutation
    one silent and one non silent S228P mutation comp…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147286-plasmid-map-sequence-id-289733

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  7. pAc5.1B-lambdaN-HA-DmCG2025_N

    Plasmid
    #146979
    Purpose
    Insect Expression of DmCG2025
    Depositor
    Elisa Izaurralde
    Insert
    DmCG2025 (CG2025 Fly)
    Expression
    Insect
    Mutation
    two silent and two non silent mutations R12G and …
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146979-plasmid-map-sequence-id-289426

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  8. pAc5.1B-EGFP-DmYTHDFa_546-700_AC

    Plasmid
    #148384
    Purpose
    Insect Expression of DmYTHDFa_546-700
    Depositor
    Elisa Izaurralde
    Insert
    DmYTHDFa_546-700 (CG6422 Fly)
    Expression
    Insect
    Mutation
    1 silent mutations compared to the sequence given…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    148384-plasmid-map-sequence-id-356619

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  9. P35m

    Plasmid
    #120946
    Purpose
    MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).
    Depositor
    Richard Murray
    Insert
    pLbda-CasRQi
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120946-plasmid-map-sequence-id-235948

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  10. mVenus-Cx26-M-7

    Plasmid
    #56608
    Purpose
    Localization: Gap Junctions, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Cx26
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    silent mutation in the untranslated sequence; GCT
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. ZBTB33A-c002

    Plasmid
    #53651
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    ZBTB33A
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    141347-plasmid-map-sequence-id-296391

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  13. G protein alpha-q-YFP

    Plasmid
    #55782
    Purpose
    This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-q EE YFP (Gnaq Mouse)
    Tags
    The EE epitope was introduced into the alpha-q se…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    55782-plasmid-map-sequence-id-119295

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  14. pMXS-mitoGshF-blasticidin

    Plasmid
    #210314
    Purpose
    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Article
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…
    Mutation
    Codon optimized for expression in human cells
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210314-plasmid-map-sequence-id-418848

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  15. pSA128 A2UCOE-HBB_IVS2-Cas9-BFP

    Plasmid
    #249152
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-Cas9-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249152-plasmid-map-sequence-id-496394

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  18. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  20. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

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Showing: 3301 - 3320 of 13590 results