We narrowed to 12,680 results for: sequence

1. A1-LCD-23S+23T

   Plasmid

   #178693

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Thr reisudes replaced with Ser (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-23S+23T (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   twenty-three Thr reisudes replaced with Ser

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. A1-LCD-10R+10K

   Plasmid

   #178671

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with ten Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-10R+10K (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   ten Arg residues replaced with Lys

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. A1-LCD-3R+3K

   Plasmid

   #178669

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with three Arg residues replaced with Lys (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-3R+3K (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   three Arg residues replaced with Lys

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. A1-LCD+7F-7Y

   Plasmid

   #178660

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD +7F-7Y (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   seven Tyr residues replaced with Phe

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. A1-LCD+12E

   Plasmid

   #178676

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD+12E (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   twelve Glu residues added

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. A1-LCD-20G+20S

   Plasmid

   #178683

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-20G+20S (HNRNPA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   twenty Gly residues replaced with Ser

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Anti-Pan-MAGUK [K28/86R]

   Plasmid

   #177440

   Purpose

   Mammalian Expression Plasmid of anti-Pan-MAGUK (Human). Derived from hybridoma K28/86.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-MAGUK (Homo sapiens) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRcCMV-STAT31F

   Plasmid

   #134725

   Purpose

   Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707

   Depositor

   Curt Horvath, Jim Darnell

   Article

   Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

   Insert

   STAT3-STAT1 Fusion (STAT3, STAT1 Human)
   

   Use

   Tags

   FLAG and human STAT3 aa 1-514 joined to human STA…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRcCMV-STAT13F

   Plasmid

   #134723

   Purpose

   Expresses chimeric STAT1-STAT3 protein with human STAT1 amino acids 1-508 joined to human STAT3 at amino acid 515, with a FLAG epitope tag at the C-terminus. Based on addgene plasmids #8691 and #8707

   Depositor

   Curt Horvath, Jim Darnell

   Article

   Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

   Insert

   STAT1-STAT3 fusion (STAT3, STAT1 Human)
   

   Use

   Tags

   FLAG and human STAT1 aa1-508 joined to human STAT…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiV_Neo_ZFP64_Delta_TAD

    Plasmid

    #118698

    Purpose

    Express ZFP64 without transcription activation domain(TAD)

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    ZFP64_Delta_TAD (ZFP64 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG was fused to the N terminal of ZFP64 cDNA

    Expression

    Mutation

    Delete amino acid from 453 to 681

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

    Plasmid

    #108372

    Purpose

    Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMIR-192-Reporter

    Plasmid

    #71872

    Purpose

    Mammalian expression vector for the analysis of miR-19α activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-192
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pMIR-193a-5p-Reporter

    Plasmid

    #71873

    Purpose

    Mammalian expression vector for the analysis of miR-193a-5p activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-193a-5p
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. CFP(1-158)-RGS7t

    Plasmid

    #55761

    Purpose

    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    RGS7(202-477)-CFP(1-158) (RGS7 Human, Aequorea victoria)
    

    Use

    Tags

    CFP(1-158) was fused to the amino terminus of RGS…

    Expression

    Mammalian

    Mutation

    The RGS sequence amino terminal to the GGL domain…

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. RGS-6xHis-LIN-29-pcDNA3.1-

    Plasmid

    #52515

    Purpose

    expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Lin-29 b (lin-29 Nematode)
    

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    wt (changed coding sequence in 5' (5th codon…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMiR-CIP2A-stop-mut

    Plasmid

    #53713

    Purpose

    Luciferase reporter assay for CIP2A with mutated firefly stop codon

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on the stop codon of firefly luciferase …

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. KHDRBS3_KH_pGEX

    Plasmid

    #135133

    Purpose

    Recombinant expression of KHDRBS3 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    KHDRBS3 (KHDRBS3 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1-IRES eGFP

    Plasmid

    #128059

    Purpose

    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-PE7-SB2

    Plasmid

    #228868

    Purpose

    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Insert

    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. hTLR2 flag

    Plasmid

    #13082

    Depositor

    Ruslan Medzhitov

    Insert

    TLR2 (TLR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-16 have been replaced with mammalia…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge