162,536 results

1. pRS418

   Plasmid

   #11256

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

3. T7 promoter + terminators reporter plasmid

   Plasmid

   #156456

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_CAG_Gag-MCP

   Plasmid

   #212637

   Purpose

   Lentivirus vector to express an MLV Gag-MCP fusion protein

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Lentiviral

   Tags

   MS2 Capsid Protein (N55K)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EndophilinA1-mRuby2

   Plasmid

   #171945

   Purpose

   Expresses Endophilin A1 in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Endophilin A1 (SH3GL2 Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cre Reporter

   Plasmid

   #62732

   Purpose

   Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   LoxP-DsRED-STOP-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   LoxP sites

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-FluoSTEP-ICUE

   Plasmid

   #181845

   Purpose

   Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. APJ-Tango

   Plasmid

   #66224

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   APJ (APLNR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-HA-LMW-FGF2

   Plasmid

   #157663

   Purpose

   Expresses FGF2 in mammalian cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-134

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCre-SacB-zeo

    Plasmid

    #107706

    Purpose

    Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Insert

    ble (Zeo resistance)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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