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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-eGFP

Plasmid

#157734

Purpose

mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-eGFP

Tags

Flag-His8

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAEMXT-deLACCO

Plasmid

#167947

Purpose

Fluorescent biosensor control for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

CD59-deLACCO-GPI

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δrev

Plasmid

#101348

Purpose

All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta rev

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 N

Plasmid

#158370

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 N (N )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV_nLightR

Plasmid

#217657

Purpose

Expresses norepinephrine indicator nLightR under mammalian promoter

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

nLightR

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-G97 IRES EGFP

Plasmid

#130711

Purpose

Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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AlkB-D135T

Plasmid

#160433

Purpose

A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

Depositor

Tao Pan

Article

Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

Insert

E. coli AlkB

Tags

His-tag

Expression

Bacterial

Mutation

Changed Aspartic acid 135 to Threonine; deleted t…

Promoter

T7

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMB3-B-TelR

Plasmid

#204992

Purpose

Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEY51

Plasmid

#191054

Purpose

mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

mec-3 (mec-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY52

Plasmid

#191055

Purpose

odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

odr-1 (odr-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

Plasmid

#101351

Purpose

HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP16

Plasmid

#158367

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP16 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf8

Plasmid

#158377

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf8 (ORF8 )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHS1398

Plasmid

#205971

Purpose

MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf7a

Plasmid

#158375

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7a (ORF7a )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHS1182

Plasmid

#205964

Purpose

NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GlycophorinE-CD4d3+4-bio

Plasmid

#73095

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin E (GYPE Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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