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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

Plasmid

#163990

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1461R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MARK3_HUMAN_D0

Plasmid

#79740

Purpose

This plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MARK3 (MARK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB1_HUMAN_D0

Plasmid

#79694

Purpose

This plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB1 (EPHB1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK3_HUMAN_D0

Plasmid

#79689

Purpose

This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK3 (STK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2G_HUMAN_D0

Plasmid

#79711

Purpose

This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2G (CAMK2G Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGST-HsSyx-375-792

Plasmid

#179292

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

His6 + GST + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 375-792 from accession number NP_0010361…

Promoter

T7

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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GAK_HUMAN_D0

Plasmid

#79682

Purpose

This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

GAK (GAK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

Plasmid

#166834

Purpose

Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

TEV-GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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KC1D_HUMAN_D0

Plasmid

#79723

Purpose

This plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1D (CSNK1D Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
CDKL1_HUMAN_D0

Plasmid

#79699

Purpose

This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDKL1 (CDKL1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Showing: 1671 - 1680 of 13134 results

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We narrowed to 13,134 results for: LIC

pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

MARK3_HUMAN_D0

EPHB1_HUMAN_D0

STK3_HUMAN_D0

KCC2G_HUMAN_D0

pGST-HsSyx-375-792

GAK_HUMAN_D0

pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

KC1D_HUMAN_D0

CDKL1_HUMAN_D0

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