We narrowed to 7,885 results for: Lif

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pPRISM-TagRFP-cmlc2-mRFP

Plasmid

#117769

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Ncam1_1.18.1-AP-His

Plasmid

#71962

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.18.1 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

Plasmid

#179435

Purpose

Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-coreHS4-mch (SC)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-MOG [L94/54]

Plasmid

#190296

Purpose

Mammalian Expression Plasmid of anti-MOG (Rat). Derived from hybridoma L94/54.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MOG (Rattus norvegicus) recombinant Mouse monoclonal antibody (Mog Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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Ncam1_0.0.0-AP-His

Plasmid

#71960

Purpose

Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_0.0.0 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Flrt3-AP-His

Plasmid

#71949

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt3 (Flrt3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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C2-82 pComb3xss anti-cFos nanobody

Plasmid

#145838

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 1-159

Plasmid

#186016

Purpose

2xHis-LRRK2 Armadillo 1-159

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)

Expression

Bacterial

Mutation

See depositor's comments below.

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 350-550

Plasmid

#186018

Purpose

2xHis-LRRK2 Armadillo 350-550

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 350-550 (LRRK2 Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-cmlc2-TagBFP

Plasmid

#117768

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 Y101A, Y103A

Plasmid

#74605

Purpose

expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Y101A, Y103A

Promoter

SFFV

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd6

Plasmid

#102868

Purpose

Expresses mouse Fzd6 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 6 (Fzd6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-gcry-mRFP

Plasmid

#117784

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-mRFP

Plasmid

#117775

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd1

Plasmid

#102864

Purpose

Expresses mouse Fzd1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 1 (Fzd1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-mCherry-SHP1 (SHP2-cSH2)

Plasmid

#180822

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its cSH2 replaced by SHP2-cSH2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Promoter

SV40

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-S100A5 [N176A/35.1R]

Plasmid

#114560

Purpose

Mammalian Expression Plasmid of anti-S100A5 (Rat). Derived from hybridoma N176A/35.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-S100A5 (Rattus norvegicus) recombinant mouse monoclonal antibody (S100a5 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-gcry-mRFP

Plasmid

#117790

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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S1-54 pComb3xss anti-cFos nanobody

Plasmid

#145836

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S1-54 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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