We narrowed to 7,952 results for: Lif

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pX459-CTNNB1-S45

Plasmid

#164587

Purpose

Expresses a gRNA that overlaps the S45 codon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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natMX6-ins5

Plasmid

#195042

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

NatR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

Plasmid

#123330

Purpose

Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-AKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-5 [N195A/16R]

Plasmid

#114481

Purpose

Mammalian Expression Plasmid of anti-QKI-5 (Human). Derived from hybridoma N195A/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-QKI-5 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SSpB-mCherry-p63DH

Plasmid

#176112

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA

Plasmid

#157750

Purpose

Expression of AURKA targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

Aurora A without the first 30AA + MTS of CoxVIII

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-mRFP

Plasmid

#117781

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-MMACHC [N230/21R]

Plasmid

#114555

Purpose

Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

Plasmid

#218882

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2(no bind)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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Flrt3-Fc-His

Plasmid

#72075

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt3 (Flrt3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

Plasmid

#188771

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

EFS

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_WT_ADRB2_LucReporter

Plasmid

#129788

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Promoter

N/A

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Ncam1_1.18.1-Fc-His

Plasmid

#72088

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.18.1 (Ncam1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRepair-mScI-CTNNB1

Plasmid

#153431

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mScarlet-i

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-CERT(PHD)(W33A)

Plasmid

#221761

Purpose

Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

CERT (CERT1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–116, mutated 33W to 33A

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-TagBFP

Plasmid

#117780

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-STAT4-P2A-mCherry

Plasmid

#203853

Purpose

Tet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

STAT4 (STAT4 Human)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL1-9155

Plasmid

#115460

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL1-9155 (MBNL1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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