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MAC-SARS-CoV-2 S

Plasmid

#158371

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 S (S )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Ensconsin-18-mEos2

Plasmid

#99230

Purpose

Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

mEos2

Tags

Ensconsin (MAP7)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag9

Plasmid

#169334

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 E

Plasmid

#158368

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 E (E )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf6

Plasmid

#158374

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf6 (ORF6 )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GAL FLAG25QDProCFPp303

Plasmid

#15584

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEY48

Plasmid

#191052

Purpose

klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

klp-6 (klp-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY47

Plasmid

#191051

Purpose

gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-21 (gcy-21 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GAL FLAG46QDProGFP p306

Plasmid

#15832

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_TLOC1 (399aa)

Plasmid

#45288

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TLOC1 (SEC62 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
LPCX Smad2 deltaSSMS

Plasmid

#12637

Depositor

Rik Derynck

Article

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

Smad2 (SMAD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Last 4 aa's deleted.

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR V2 sgMTHFD1L_1

Plasmid

#106316

Purpose

Express Cas9 and sgRNA targeting MTHFD1L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD1L

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP1

Plasmid

#158352

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP1 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Mutation

ARS sequence mutated to remove sapI site for dome…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe-PI(WT)-Src(KD)-mCherry

Plasmid

#87358

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

D388R

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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GAL 25Q+ProGFPp416

Plasmid

#15580

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEY15

Plasmid

#191021

Purpose

flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-1 (flp-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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GAL 46Q+ProGFPp416

Plasmid

#15581

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q + Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGEX SHIP2-SH2

Plasmid

#46493

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP2

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-131

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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