We narrowed to 6,371 results for: siae

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pCfB2797

Plasmid

#63639

Purpose

pCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2875

Plasmid

#63640

Purpose

pCfB2875 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2989

Plasmid

#63636

Purpose

pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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ISceI-GR-RFP

Plasmid

#17654

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

I-SceI-GRLBD (SCEI, Nr3c1 Budding Yeast, Rat)

Tags

mRFP

Expression

Mammalian

Mutation

C655G mutation in the glucocorticoid receptor (se…

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pCfB2188-PrARG8-GFP::URA3

Plasmid

#216512

Purpose

Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrARG8-yeGFP

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

ARG8

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB2197-PrGNP1-mK2-NatMX

Plasmid

#216513

Purpose

Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

Depositor

Juha Saarikangas

Article

Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

Insert

PrGnp1-mKate2

Use

Cre/Lox; Integrative

Expression

Yeast

Promoter

GNP1

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCS_FLPER(T2)

Plasmid

#31303

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP-ER ligand binding domain (ESR1 Budding Yeast, Human)

Expression

Mammalian

Mutation

full length FLP linked to ligand binding domain o…

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEK39

Plasmid

#210599

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter

Tags

mRuby3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Tags

mCerulean3

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM20C

Plasmid

#135487

Purpose

Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr H2B-tdTomato

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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p403-BrdU-Inc

Plasmid

#71789

Purpose

BrdU-incorporating plasmid with selectable marker HIS3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Expression

Yeast

Promoter

tetO7 promoter

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

Plasmid

#68280

Purpose

SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Mkk2DS NLS mCherry (MKK2 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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EC2_10_dCas9_VPR_HC_sgRNA

Plasmid

#163712

Purpose

Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VPR

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMK425 (GAL-OsTIR1(F74A))

Plasmid

#140657

Purpose

GAL-OsTIR1(F74A)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74A)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#78231

Purpose

Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Cas9

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMK198 (GAL-OsTIR1)

Plasmid

#140655

Purpose

GAL-OsTIR1(WT)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMT010

Plasmid

#64762

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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p306-BrdU-Inc

Plasmid

#71792

Purpose

BrdU-incorporating plasmid with selectable marker URA3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA-ura-HYB

Plasmid

#64330

Purpose

ura3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of ura3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMT006

Plasmid

#64761

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Tags

mScarlet fused to FKBP

Expression

Yeast

Promoter

SP6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDS240

Plasmid

#34966

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

K533∆, high-affinity epitope

Promoter

GAL1

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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