We narrowed to 10,818 results for: HA

1. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

   Plasmid

   #112683

   Purpose

   An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   iRFP713
   

   Use

   AAV

   Tags

   2A-iCre and Nuclear localization signal

   Expression

   Mutation

   Promoter

   CMV-IE

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hras (G12V)-pcw107

   Plasmid

   #64603

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

   Plasmid

   #188901

   Purpose

   dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   SID-dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

   Plasmid

   #112719

   Purpose

   An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Mecp2

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

   Plasmid

   #47633

   Purpose

   An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   hChR2 (H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   EF1a

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-H-RAS V12

   Plasmid

   #31201

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   H-Ras (HRAS Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   G12V constitutively active

   Promoter

   Available Since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pIG-703pUC19-tNGFR-P2A-BACH2

   Plasmid

   #186112

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BACH2 (BACH2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-712pUC19-tNGFR-P2A-ITCH

   Plasmid

   #186085

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   ITCH (ITCH Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTR-177 pUC19-CLTA-N-mCherry

   Plasmid

   #186128

   Purpose

   knockin of mCherry to CLTA target

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CLTA (CLTA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with mCherry sequence

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTR-338 pUC19-tNGFR-P2A-LAG3 N

    Plasmid

    #186055

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    LAG3 (LAG3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTR-469 JUNB-tNGFR

    Plasmid

    #186060

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    JUNB (JUNB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTR-472 FOXO1-tNGFR

    Plasmid

    #186062

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIG-721 CTLA4-GFP T124P

    Plasmid

    #186126

    Purpose

    CTLA4 gene knockin with mutation

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    T124P

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIG-648 CTLA4int1-CTLA4-GFP-SV40

    Plasmid

    #186123

    Purpose

    CTLA4 gene knockin

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with GFP sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

    Plasmid

    #70076

    Purpose

    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Silent mutations are introduced to escape from RN…

    Promoter

    Tet responsible promoter

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDECKO_GFP_AAVS1

    Plasmid

    #141211

    Purpose

    pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTR-374 tNGFR-P2A-B2M

    Plasmid

    #186068

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDECKO_mCherry_AAVS1_1801

    Plasmid

    #159090

    Purpose

    targeting, non-essential locus control

    Depositor

    Rory Johnson

    Article

    Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

    Insert

    gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid

    #70075

    Purpose

    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Y102A. Silent mutations are introduced to escape …

    Promoter

    Tet responsible promoter

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIG-709pUC19-tNGFR-P2A-IL10

    Plasmid

    #186117

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL10 (IL10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIG-746pUC19-tNGFR-P2A-CD5

    Plasmid

    #186121

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD5 (CD5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pIG-738pUC19-tNGFR-P2A-Kindlin-3

    Plasmid

    #186092

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    KINDLIN3 (FERMT3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. R713-M50-303: CMV51p> Halotag-HRasHVR

    Plasmid

    #159685

    Purpose

    Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-HRasHVR (HRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIG-736pUC19-tNGFR-P2A-CD40L

    Plasmid

    #186104

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD40L (CD40LG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTR-471 IRF4-tNGFR

    Plasmid

    #186061

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IRF4 (IRF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p3E mCherry-T2A-hsHRAS-G12V-pA (JDW 1188)

    Plasmid

    #242571

    Purpose

    Gateway 3' entry clone containing mCherry followed by a T2A cleavage peptide and then human HRAS G12V.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    HRAS-G12V (HRAS Human)
    

    Use

    Gateway subcloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TOPO-EXOC3 (-1557 to-1046)

    Plasmid

    #226446

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1557 to-1046) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1557 to-1046) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TRE-RXRa-Myc; pGK-H2B-RFP

    Plasmid

    #242887

    Purpose

    For lentiviral overexpression of Myc-tagged human RXRa coupled to H2B-RFP (in the opposite direction)

    Depositor

    Elaine Fuchs

    Article

    Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

    Insert

    Rxra (RXRA Human)
    

    Use

    Lentiviral

    Tags

    H2B-mRFP

    Expression

    Mammalian

    Mutation

    Human RXRa cDNA was PCR-amplified from pSV-Sport-…

    Promoter

    Tetracycline response element upstream of a minim…

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TOPO-EXOC3 (-2455 to -1951)

    Plasmid

    #226441

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -2455 to -1951) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-2455 to -1951) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TOPO-EXOC3 (-1592 to -1046)

    Plasmid

    #226444

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1592 to -1046) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1592 to -1046) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TOPO-EXOC3 (-1591 to -1009)

    Plasmid

    #226443

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1591 to -1009) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1591 to -1009) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TOPO-EXOC3 (-1701 to -1594)

    Plasmid

    #226442

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1701 to -1594) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1701 to -1594) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TOPO-EXOC3 (-1592 to -1444)

    Plasmid

    #226445

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1592 to -1444) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1592 to -1444) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TOPO-EXOC3 (-1557 to-1444)

    Plasmid

    #226447

    Purpose

    For subcloning of human EXOC3 promoter (base pairs -1557 to-1444) or for assays using M13 phage

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    EXOC3 (-1557 to-1444) (EXOC3 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    No

    Promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJL-mGold2t

    Plasmid

    #231762

    Purpose

    Expression of mGold2t in yeast cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    pTDH(GAP promoter)

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. sgLATS1/2-1

    Plasmid

    #229431

    Purpose

    knockout of LATS1 and LATS2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgLATS1/2-1 (LATS1, LATS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge