We narrowed to 10,180 results for: yeast
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Plasmid#212698PurposegRNA targeting to the intergradtion site E4DepositorInsertgRNA targeting to the intergradtion site E4
ExpressionYeastAvailable SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Tgl3-4V5-APEX2-URA
Plasmid#207059PurposeExpressing Tgl3-4V5-APEX2. Insufficient signal for EM.DepositorInsertTgl3
Tags4xV5-APEX2ExpressionYeastPromoterpTGL3Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Pex3-4V5-APEX2-URA
Plasmid#207057PurposeExpressing Pex3-4V5-APEX2. Insufficient signal for EM.DepositorInsertPEX3
Tags4xV5-APEX2ExpressionYeastPromoterpPEX3Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Atg32-deltaPsR pXP721
Plasmid#200735PurposePlasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.DepositorInsertAtg32
TagsGFPExpressionYeastMutationAmino acids 200-341 are deleted.Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_15_UPRpro_X2site
Plasmid#194427PurposePlasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein ResponseDepositorInsertUPRpro
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT2_32_PyruEth_X2site
Plasmid#194428PurposePlasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)DepositorInsertPyruEth
UseUsed as donor dna in genome integration after lin…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_75_Donor_Tag_RPL13A-mTurq2
Plasmid#194415PurposePlasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRproDepositorInsertmTurquoise2 CDS
UseSynthetic Biology; Used as donor dna in genome in…ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.004
Plasmid#184969PurposeNegative control, empty integrating inducible casetteDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Kan
Plasmid#188928PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Kan
ExpressionYeastAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-URA
Plasmid#188927PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Phleomycin
Plasmid#188925PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Phleomycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Hygromycin
Plasmid#188924PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Hygromycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-Phleomycin
Plasmid#188920PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Phleomycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-Hygromycin
Plasmid#188919PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Hygromycin
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB520
Plasmid#185102Purpose1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused to HA and GFPDepositorInsertPOM152
TagsGFPExpressionYeastMutationPOM152(1-200)-GFPAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-TalO8 (Blank-CoTrIP)
Plasmid#178303PurposeBlank CoTrIP plasmidDepositorTypeEmpty backboneTagsNAExpressionYeastPromoterNAAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB521
Plasmid#185103Purpose1st 960 amino acids (C-terminal truncation) of Pom152 fused with HA and GFPDepositorInsertPOM152
TagsGFPExpressionYeastMutationPOM152(1-960)-HA-GFPAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#174066PurposeEncodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal1-LacO-3NLS-CIB1-VP16-tADH1
TagsFLAGExpressionYeastPromoterpGal1Available SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-NoBS-NoPseudoBS
Plasmid#100986PurposeRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
ExpressionBacterial and YeastMutationBS mutated to GTTAGTG, pseudo BS mutated to GTTTG…Available SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL45
Plasmid#174067PurposeExpresses CIB1 in S. cerevisiae. Includes inducible lac operon.DepositorInsertpGal1-LacO-3NLS-CIB1-tADH1
TagsFLAGExpressionYeastPromoterpGal1Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#174070PurposeExpresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal-Olac-3xNLS-VP16-CIB1-tADH1
TagsFLAGExpressionYeastPromoterpGal1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-U57A
Plasmid#169927PurposeWT U6 with a U57A mutationDepositorInsertU6 U57A
ExpressionBacterial and YeastAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG97
Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
ExpressionYeastAvailable SinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI743 dual-barcoded gRNA parent vector
Plasmid#164915PurposeCEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid librariesDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GXB
Plasmid#160926PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)DepositorInsertsBFP
sfGFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyExpressionYeastPromoteract1Available SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1tetAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJPS2957
Plasmid#135591PurposeSNR6 has GAGAUUUAUUUC at position 96–107 deletedDepositorInsertsnR6 (snR6 Budding Yeast)
ExpressionYeastMutationU6Δ12 has GAGAUUUAUUUC at position 96–107 deletedPromoterNativeAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GGE139
Plasmid#120754PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 2 (P2_EYK300-4AB)DepositorInsertP2_EYK300-4AB
UseCloning/assembly plasmidAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW294
Plasmid#139491PurposeInsertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGRE2 24xPP7sl
ExpressionYeastMutationWTPromoterpGRE2Available SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
ExpressionYeastMutationWTPromoterpSTL1Available SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW295
Plasmid#139492PurposeInsertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpALD3 24xPP7sl
ExpressionYeastMutationWTPromoterpALD3Available SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP266
Plasmid#139488PurposeInsertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpCTT1 24xPP7sl
ExpressionYeastMutationWTPromoterpCTT1Available SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP264
Plasmid#139487PurposeInsertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl
ExpressionYeastMutationWTPromoterpSTL1Available SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW200
Plasmid#139489PurposeInsertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGPD1 24xPP7sl
ExpressionYeastMutationWTPromoterpGPD1Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
GGE207
Plasmid#120792PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assemblyDepositorInsertInsDown-Mfe
UseCloning/assembly plasmidAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE108
Plasmid#120753PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 2 (P2_EYK300-3AB)DepositorInsertP2_EYK300-3AB
UseCloning/assembly plasmidAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE261
Plasmid#120783PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 3 (G3_mTurquoise)DepositorInsertG3_mTurquoise
UseCloning/assembly plasmidAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE070
Plasmid#120782PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 2 (G2_YFP)DepositorInsertG2_YFP
UseCloning/assembly plasmidAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE275
Plasmid#120763PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 3 (P3_EYK300-4AB)DepositorInsertP3_EYK300-4AB
UseCloning/assembly plasmidAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE273
Plasmid#120762PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position 3 (P3_EYK300-3AB)DepositorInsertP3_EYK300-3AB
UseCloning/assembly plasmidAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only