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pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMT2 GATA1

Plasmid

#13626

Depositor

Anjana Rao

Publication

Monticelli et al J Biol Chem. 2004 Aug 27. 279(35):36210-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pGEX-2T-Rac1-Q61L

Plasmid

#12979

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

Q61L Constitutively active

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
1257 pGEX4T3 GST FKHR

Plasmid

#10706

Depositor

William Sellers

Insert

FKHR (FOXO1 Human)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSuperRetro control

Plasmid

#11189

Depositor

Bruce Spiegelman

Publication

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pLY239

Plasmid

#184152

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R3

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBACgus N-His/GST

Plasmid

#40399

Depositor

Michael J Fox Foundation MJFF

Insert

GST

Use

Baculovirus

Tags

HisTev

Mutation

WT

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pDS_P(dsCsgA)-dsCsgA-Mfp3-S

Plasmid

#241785

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-S

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_P(aphII)-dsCsgA-Mfp3-S

Plasmid

#241782

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-S

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 S/ArotoL

Plasmid

#231880

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-S_DM

Plasmid

#217187

Purpose

Mammalian expression vector for expression of S_DM transposase (CMV promoter)

Depositor

Yong E Zhang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

S_DM transposase

Expression

Mammalian

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 HCoV-NL63 S_stop

Plasmid

#168682

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

S (HCNV63gp2 )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223 HCoV-NL63 S_nostop

Plasmid

#168664

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

S (HCNV63gp2 )

Use

Other

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pCEP4-myc-S (P.1 lineage / Brazilian variant)

Plasmid

#169847

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tag

Depositor

Erik Procko

Publication

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (B.1.1.7 lineage / UK variant)

Plasmid

#169848

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tag

Depositor

Erik Procko

Publication

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA

Plasmid

#191218

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Beta (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

D80A D215G (Del 241-243) K417N E484K N501Y D614G …

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-2P-Omicron-BA.5

Plasmid

#213828

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant

Depositor

Priyamvada Acharya

Publication

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pαH-S-GSAS/Omicron-2P-BA5 (S )

Tags

HRV 3C cleavage site (before tags) 8X His tag 2X…

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-S-B.1.1.7-FLAG

Plasmid

#177093

Purpose

B.1.1.7 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein B.1.1.7 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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