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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S285A/T289A-3xFLAG

Plasmid

#69841

Purpose

This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S285A/T289A

Promoter

CMV

Available since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 MMP2 g1

Plasmid

#170824

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 MMP2 g2

Plasmid

#170825

Purpose

PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d MMP2 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

Plasmid

#170826

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

Plasmid

#170827

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

Plasmid

#170828

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1

Plasmid

#170822

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA1

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mEGFP-Gphn_P1

Plasmid

#194978

Purpose

AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mEGFP-Gphn (isoform P1) (Gphn Synthetic, Rat)

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-GFP-LA

Plasmid

#206198

Purpose

Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Lmna (LMNA Human)

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

Tnnt2

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-LA

Plasmid

#206197

Purpose

Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Lmna (LMNA )

Use

AAV

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Tags

Expression

Mutation

N/A

Promoter

CAG

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-1_Xt[EF1α-copGFP]

Plasmid

#218325

Purpose

Donor plasmid of Tc1-1_Xt Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-3_FR[EF1α-copGFP]

Plasmid

#218326

Purpose

Donor plasmid of Tc1-3_FR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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