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GGE001

Plasmid

#120740

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_PGM)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_PGM

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-pomMx1

Plasmid

#30536

Purpose

Reporter (luciferase) for interferon signalling in fish

Depositor

Bertrand Collet, Christopher Secombes

Article

Collet et al Dev Comp Immunol. 2004 Jun . 28(7-8):793

Insert

rainbow trout Mx 1 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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GGE080

Plasmid

#120774

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T3_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGD431

Plasmid

#132382

Purpose

contains the sequence of mRuby3for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby3

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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MMTV-GAL4-SV40

Plasmid

#19255

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Insert

GAL4

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pEB1-comCerulean3

Plasmid

#104017

Purpose

Plasmid encoding comCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as mCerulean3 (codon optimi…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PAN

Plasmid

#200971

Purpose

Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Insert

PAN

Tags

6xHis and 6xHis-thrombin site

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHES834

Plasmid

#87947

Purpose

Expresses mKate2 under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mKate2

Use

Synthetic Biology

Expression

Yeast

Promoter

pGal1

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGD250

Plasmid

#132371

Purpose

contains the sequence of mClover3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mClover3

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm) -R

Plasmid

#48888

Purpose

Negative feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGMCZq17-OXOX

Plasmid

#31289

Depositor

Dirk Schnappinger

Article

Venugopal et al Cell Host Microbe. 2011 Jan 20. 9(1):21-

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
GGE176

Plasmid

#120733

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_Lys5)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

Marker_Lys5

Use

Cloning/assembly plasmid

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-coCeruleanME

Plasmid

#104016

Purpose

Plasmid encoding coCeruleanME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coCeruleanME

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Cerulean ME (codon optim…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKate2-T2A-mTurquoise2

Plasmid

#118426

Purpose

Produces equimolar expression of mKate2 and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mKate2-T2A-mTq2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GGE014

Plasmid

#120765

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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cyterm-ymScarletI pDRF1-GW

Plasmid

#168082

Purpose

Expresses Cyterm-ymScarletI to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymScarletI

Expression

Yeast

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW eGFP-T2A-mCherry

Plasmid

#118422

Purpose

Produces equimolar expression of eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

eGFP-T2A-mCherry

Expression

Yeast

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa-F

Plasmid

#101193

Purpose

A fast switching Dronpa variant

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

ffDronpa-F

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

K45I, V60A, F173V

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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GGE257

Plasmid

#120771

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Xpr2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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