We narrowed to 12,680 results for: sequence

1. pCIneo-lambdaN-HA-HsSmg7_1-632_I

   Plasmid

   #146532

   Purpose

   Mammalian Expression of HsSmg7_1-632

   Depositor

   Elisa Izaurralde

   Insert

   HsSmg7_1-632 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three silent mutation G216A, T1185C and A1521G co…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-HsTNRC6A_C-term_F

   Plasmid

   #146258

   Purpose

   Insect Expression of HsTNRC6A_Cterm

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A_Cterm (TNRC6A Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation K1578E compared to the se…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-Dm4EHP-A_G

   Plasmid

   #146346

   Purpose

   Insect Expression of Dm4EHP-A

   Depositor

   Elisa Izaurralde

   Insert

   Dm4EHP-A (4EHP Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   4 silent mutations compared to the sequence from …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIneo-lambdaN-HA-HsTNRC6A_1-1609_H

   Plasmid

   #146440

   Purpose

   Mammalian Expression of HsTNRC6A_1-1609

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A_1-1609 (TNRC6A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A339T, R934G, D1289G, K1578E compared to the sequ…

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmAUB_A

   Plasmid

   #145851

   Purpose

   Insect Expression of DmAUB

   Depositor

   Elisa Izaurralde

   Insert

   DmAUB (aub Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two silent mutations compared to the sequence giv…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. UC16m

   Plasmid

   #121038

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gQi Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. UC17m

   Plasmid

   #121039

   Purpose

   MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gV1 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREP81xV5_ccdB

   Plasmid

   #79019

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREP41xV5_ccdB

   Plasmid

   #79017

   Purpose

   Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

   Depositor

   Pernilla Bjerling, Göran Akusjärvi

   Type

   Empty backbone

   Use

   Tags

   double V5 epitope tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREP82xV5_ccdB

    Plasmid

    #79020

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Use

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREP42xV5_ccdB

    Plasmid

    #79018

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

    Depositor

    Pernilla Bjerling, Göran Akusjärvi

    Type

    Empty backbone

    Use

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mVenus-Paxillin-KM-N-18

    Plasmid

    #56334

    Purpose

    Localization: Focal Adhesions, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    Paxillin KM
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Mutation to remove Kozak sequence

    Promoter

    CMV

    Available since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. p(+)MV-NSE-FlagP-F497D-3p

    Plasmid

    #58797

    Purpose

    Expresses recombinant measles virus with Flag-P-F497D gene tagged si2 (siP) target sequence

    Depositor

    Branka Horvat

    Insert

    Measles virus antigenome
    

    Use

    Tags

    Flag

    Expression

    Mutation

    MeV-P F497D

    Promoter

    T7

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLXRN-EGFP

    Plasmid

    #99207

    Purpose

    EGFP expressing bicistronic retroviral vector

    Depositor

    Peter Pedersen

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A Kozak sequence was incorporated to enhance tran…

    Promoter

    LTR

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. ORFtag_TetR-SD_Frame1

    Plasmid

    #220981

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Minos ITR loxp MCS lox2272

    Plasmid

    #218983

    Purpose

    Plasmid containing ITR sequences compatible with Minos transposase and multiple cloning site

    Depositor

    Amro Hamdoun

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mTLR4 flag

    Plasmid

    #13087

    Depositor

    Ruslan Medzhitov

    Insert

    TLR4 (Tlr4 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino acids 1-19 have been replaced with mammalia…

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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