Addgene

Showing: 321 - 340 of 13545 results

Results per page:

Sort results by:

pcDNA3-MAVS-M1A-invitro(KaganE12)

Plasmid

#52016

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-G67M-invitro(KaganD100)

Plasmid

#52014

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Gly 67 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-L62M-invitro(KaganD98)

Plasmid

#52013

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Leu 62 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-E80M-invitro(KaganD99)

Plasmid

#52015

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Glu 80 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M142A-invitro(E13)

Plasmid

#52017

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

Plasmid

#87586

Purpose

PV7 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

Plasmid

#50896

Purpose

Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and YFP

Expression

Mammalian

Mutation

L717C C723S C724V in hNKCC1 in NT17

Promoter

CMV

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry

Plasmid

#89057

Purpose

PV4 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

GFP-T2A-mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

Plasmid

#87574

Purpose

PV1 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T-Mce3R_K262A

Plasmid

#225728

Purpose

To express Mce3R mutant K262A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

K262A

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelG

Plasmid

#225729

Purpose

To express MelG (Rv1937) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-VapBC35

Plasmid

#225733

Purpose

To express VapBC35(Rv1962) Toxin antitoxin in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

vapBC35

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T_Mce3R

Plasmid

#225726

Purpose

To express Mce3R in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-TrxC

Plasmid

#225742

Purpose

To express TrxC(Rv3914) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxC

Use

Tags

6xHis

Expression

Bacterial

Mutation

TrxC

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-TrxB2

Plasmid

#225741

Purpose

To express TrxB2(Rv3913) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxB2

Use

Tags

6xHis

Expression

Bacterial

Mutation

TrxB2

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelI

Plasmid

#225730

Purpose

To express MelI (Rv1939) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melI

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelK

Plasmid

#225732

Purpose

To express MelK (Rv1941) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melK

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-MelJ

Plasmid

#225731

Purpose

To express MelJ (Rv1940) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melJ

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge