We narrowed to 753 results for: PI

1. pAT9753-BEAR-mScarlet-preedited

   Plasmid

   #162994

   Purpose

   BEAR control plasmid with split mScarlet and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mScarlet split with an intron between amino acids 110-111
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA FLAG p85beta

   Plasmid

   #237336

   Purpose

   transient overexpression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

   Insert

   p85beta (PIK3R2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFNC-2

   Plasmid

   #227667

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. KmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   KmR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIFR-5

   Plasmid

   #233294

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   p14G-BCD2

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIFR-8

   Plasmid

   #227666

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   AmR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIFR-6

   Plasmid

   #227665

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p14G-BCD2

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIFR-4

   Plasmid

   #227664

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p14G-BCD2

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIFR-2

   Plasmid

   #227663

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   p14G-BCD2

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFNC-8

    Plasmid

    #227670

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    AmR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFNC-4

    Plasmid

    #227668

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    SmR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL435

    Plasmid

    #226628

    Purpose

    pcDNA-dTdT

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    catalytically deactivated TdT (DNTT Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D343E, D345E

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS44i8GH-1

    Plasmid

    #207525

    Purpose

    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKH-1699

    Plasmid

    #124227

    Purpose

    Mammalian SpCas9-HF1 expression

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWN10042

    Plasmid

    #89052

    Purpose

    GF-ori-FP plasmid for GFxFP assay

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    EGFP part 1
    EGFP part 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and none

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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