Skip to main content
Addgene
Showing: 641 - 680 of 4182 results

  2. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75349-plasmid-map-sequence-id-266810

    Enlarge

  9. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid
    #128256
    Purpose
    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.
    Depositor
    Gabor Balazsi
    Article
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Insert
    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128256-plasmid-map-sequence-id-248948

    Enlarge

  13. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

    Enlarge

  14. pEND-443_Cacnes_HisB

    Plasmid
    #225608
    Purpose
    Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225608-plasmid-map-sequence-id-451366

    Enlarge

  15. pEND-446_Cacnes_ProA

    Plasmid
    #225609
    Purpose
    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225609-plasmid-map-sequence-id-451370

    Enlarge

  16. pEND-320_Cacnes_ori

    Plasmid
    #225602
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225602-plasmid-map-sequence-id-451377

    Enlarge

  23. pJL NarL YdfI Flag

    Plasmid
    #225650
    Purpose
    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NarL-YdfI (narL Synthetic)
    Use
    Cell free expression
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    NarL with native DNA-binding domain replaced with…
    Promoter
    T7
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    225650-plasmid-map-sequence-id-454890

    Enlarge

  25. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225649
    Purpose
    tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA, araBAD and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225649-plasmid-map-sequence-id-448716

    Enlarge

  26. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

    Enlarge

  33. pEND-227_Cacnes_mCerulean

    Plasmid
    #225614
    Purpose
    Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mCerulean
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225614-plasmid-map-sequence-id-451417

    Enlarge

  34. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

    Enlarge

  35. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75347-plasmid-map-sequence-id-232926

    Enlarge

  37. pEND-184_Cacnes_mCherry

    Plasmid
    #225610
    Purpose
    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225610-plasmid-map-sequence-id-451420

    Enlarge

  38. pEND-229_Cacnes_EforCP

    Plasmid
    #225615
    Purpose
    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    EforCP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225615-plasmid-map-sequence-id-451383

    Enlarge

  39. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

    Enlarge

  40. pEND-303_Cacnes_tetR

    Plasmid
    #225618
    Purpose
    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    TetR
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225618-plasmid-map-sequence-id-451367

    Enlarge

Showing: 641 - 680 of 4182 results