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Showing: 641 - 680 of 975 results

  1. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  3. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid
    #172915
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7f-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172915-plasmid-map-sequence-id-341221

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  5. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

    Plasmid
    #172916
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7s-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172916-plasmid-map-sequence-id-341478

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  7. AiP12546 - pAAV-AiE0050m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN2546)

    Plasmid
    #224060
    Purpose
    AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    tdTomato
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    224060-plasmid-map-sequence-id-450093

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  10. pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183025
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183025-plasmid-map-sequence-id-360497

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  11. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid
    #172914
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7c-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172914-plasmid-map-sequence-id-341479

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  12. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid
    #172913
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7b-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    172913-plasmid-map-sequence-id-341198

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  14. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51094
    Purpose
    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    oChIEF(E163A/T199C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E163A/T199C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51094-plasmid-map-sequence-id-79315

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  15. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51095
    Purpose
    Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    ChIEF(E162A/T198C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E162A/T198C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51095-plasmid-map-sequence-id-79317

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  16. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Expression
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  17. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

    Plasmid
    #172918
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7c-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172918-plasmid-map-sequence-id-343805

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  21. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

    Plasmid
    #172917
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7b-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172917-plasmid-map-sequence-id-343804

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  22. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

    Plasmid
    #172920
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7s-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172920-plasmid-map-sequence-id-343802

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  23. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

    Plasmid
    #172919
    Purpose
    LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7f-T2A-tdTomato
    Use
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Mutation
    Promoter
    hsp70
    Available since
    Sept. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172919-plasmid-map-sequence-id-343801

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  25. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183027
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183027-plasmid-map-sequence-id-360504

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  27. AAV-Syn-NS1

    Plasmid
    #175276
    Purpose
    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    NLS-dTomato (P2A cleavage)
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175276-plasmid-map-sequence-id-349086

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  28. pMTB-Multibow-R

    Plasmid
    #60983
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic dTomato
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    dTomato
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pMTB-Multibow-hR

    Plasmid
    #60997
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    dTomato
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pMTB-Multibow-mR

    Plasmid
    #60990
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomato
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    dTomato
    Use
    Zebrafish
    Tags
    membrane tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. SiC-V1-Scr

    Plasmid
    #133042
    Purpose
    SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133042-plasmid-map-sequence-id-264423

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  35. pUltra-Chili

    Plasmid
    #48687
    Purpose
    3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    48687-plasmid-map-sequence-id-70485

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  37. pLenti- V6.3 Ultra-Chili

    Plasmid
    #106173
    Purpose
    lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable
    Depositor
    Ewa Snaar-Jagalska
    Insert
    dTomato
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106173-plasmid-map-sequence-id-207442

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Showing: 641 - 680 of 975 results