-
Plasmid#112912PurposeExpress the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tagDepositorInsertdematin (DMTN Human)
UseTagsGlutatione-S-Tranferase and precision protease si…ExpressionBacterialMutationPEST sequence near the N-terminal removed. 5′-GCG…PromoterT7Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAT6
Plasmid#112594PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneUseTags6xHis-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAT5
Plasmid#112593PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneUseTags6xHis-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAT3
Plasmid#112584PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneUseTags6HisExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)
Plasmid#112803PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 554_557 deleted/PDZPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES945
Plasmid#112643Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertCRY2PHR-GAL4DBD
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES 946
Plasmid#112644Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertRTG3AD-GSx7-CIB1-SV40NLS (CIB1 Mustard Weed, Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationPromoterADH1Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
Plasmid#112802PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 14_22 and 447_454 deletedPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta14_22)
Plasmid#112800PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 14_22 deletedPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES944
Plasmid#112642Purposeexpress SST2 from the GAL1 promoterDepositorInsertSST2
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-yckABD
Plasmid#112790PurposeDownstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilisDepositorArticleInsertyckABD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-yckABU
Plasmid#112789PurposeUpstream homology arm, Pair of HAs spans yckA and yckB, which share homology with ABC transporters in B. subtilisDepositorArticleInsertyckABU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-spsCD
Plasmid#112788PurposeDownstream homology arm, SpsC is involved in spore coat polysaccharide synthesisDepositorArticleInsertspsCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-spsCU
Plasmid#112787PurposeUpstream homology arm, SpsC is involved in spore coat polysaccharide synthesisDepositorArticleInsertspsCU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-rrnOU
Plasmid#112785PurposeUpstream homology arm, rrnO-23S (23S rRNA)DepositorArticleInsertrrnOU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-recJU
Plasmid#112783PurposeUpstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenanceDepositorArticleInsertrecJU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-C-cat
Plasmid#112791PurposeChloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)DepositorArticleInsertcat
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-rrnOD
Plasmid#112786PurposeDownstream homology arm, rrnO-23S (23S rRNA)DepositorArticleInsertrrnOD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-D-amyED
Plasmid#112782PurposeDownstream homology arm, amyE encodes _-amylaseDepositorArticleInsertamyED
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-amyEU
Plasmid#112781PurposeUpstream homology arm, amyE encodes _-amylaseDepositorArticleInsertamyEU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-PliaG
Plasmid#112777PurposeWeak constitutive promoter of liaGFSR operon involved in detection of cell wall damageDepositorArticleInsertPliaG promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 P294A
Plasmid#112102PurposeMammalian expression of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationP294APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 R290A D291A T292A
Plasmid#112103PurposeMammalian expression of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationR290A D291A T292APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 T292A L293A P294A
Plasmid#112104PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationT292A L293A P294APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 L330R Q334R L702R
Plasmid#112105PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationL330R Q334R L702RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 T78R L84R T92R V118R
Plasmid#112106PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationT78R L84R T92R V118RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406A M407A
Plasmid#112107PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406A M407APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406A M407A F409A
Plasmid#112108PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406A M407A F409APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R
Plasmid#112109PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406R M407RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R F409A
Plasmid#112110PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406R M407R F409APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R T488W
Plasmid#112111PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406R M407R T488WPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJPN0003
Plasmid#112080PurposeGFP module NT2DepositorInsertGFP
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE1001
Plasmid#112079PurposeOverexpression of the AtCAS9 in plant under 2x35S promoterDepositorInsertAtCAS9
UseCRISPRTagsExpressionPlantMutationPlant codon optimized Cas9 S. pyogenesPromoter2x35SAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORFE1052
Plasmid#112078PurposeOverexpression of the AtCAS9 nickase in plant under 2x35S promoterDepositorInsertAtCAS9 D10A
UseCRISPRTagsExpressionPlantMutationPlant codon optimized nCas9 D10A S. pyogenesPromoter2x35SAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJPN0018
Plasmid#112083PurposeeCFP module CTDepositorInserteCFP
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3
Plasmid#112843Purposetransient/stable overexpression of the C-terminal domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationC2 domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1
Plasmid#112841Purposetransient/stable overexpression of the N-terminal domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationN domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
UseTags6His-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
UseTags6His-TEVExpressionBacterialMutationR47APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
UseTags6His-TEVExpressionBacterialMutationR47KPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
UseTags6His-TEVExpressionBacterialMutationQ48APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
UseTags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
UseTags6His-TEVExpressionBacterialMutationT50APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
UseTags6His-TEVExpressionBacterialMutationP51APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
UseTags6His-TEVExpressionBacterialMutationR52APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
UseTags6His-TEVExpressionBacterialMutationQ54APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(delta C-term)
Plasmid#112731PurposeThis mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.DepositorInsert6His-TEV-TBP6.7(delta C-term)
UseTags6His-TEVExpressionBacterialMutationdelta C-termPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAP-Amotl2
Plasmid#112834Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsTAPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/LL5BIP-GFP
Plasmid#112831Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only